Huang Dabing, Zhu Xianhai, Wang Yong, Yu Haobin, Pu Youguang
Department of Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, Anhui Province, P.R. China.
Department of Interventional Oncology, Anhui Provincial Cancer Hospital, West Branch of the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, Anhui Province, P.R. China.
Aging (Albany NY). 2020 Sep 5;12(17):16936-16950. doi: 10.18632/aging.103600.
Long non-coding RNAs (lncRNAs) were found to play roles in various cancers, including nasopharyngeal carcinoma. In this study, we focused on the biological function of the lncRNA FAM133B-2 in the radio-resistance of nasopharyngeal carcinoma. The RNA-seq and qRT-PCR analysis showed that FAM133B-2 is highly expressed in the radio-resistant nasopharyngeal carcinoma cells. The following biochemical assays showed that FAM133B-2 represses the nasopharyngeal carcinoma radio-resistance and also affects the apoptosis and proliferation of nasopharyngeal carcinoma cells. Further investigations suggested that miR-34a-5p targets FAM133B-2 and also regulates the cyclin-dependent kinase 6 (CDK6). All these results suggested that the lncRNA FAM133B-2 might function as a competitive endogenous RNA (ceRNA) for miR-34a-5p in nasopharyngeal carcinoma radio-resistance, thus it may be regarded as a novel prognostic biomarker and therapeutic target in nasopharyngeal carcinoma diagnosis and treatment.
长链非编码RNA（lncRNAs）被发现参与多种癌症，包括鼻咽癌。在本研究中，我们聚焦于lncRNA FAM133B-2在鼻咽癌放射抗性中的生物学功能。RNA测序和qRT-PCR分析表明，FAM133B-2在放射抗性的鼻咽癌细胞中高表达。随后的生化分析表明，FAM133B-2抑制鼻咽癌的放射抗性，并影响鼻咽癌细胞的凋亡和增殖。进一步研究表明，miR-34a-5p靶向FAM133B-2并调节细胞周期蛋白依赖性激酶6（CDK6）。所有这些结果表明，lncRNA FAM133B-2在鼻咽癌放射抗性中可能作为miR-34a-5p的竞争性内源性RNA（ceRNA）发挥作用，因此它可能被视为鼻咽癌诊断和治疗中的一种新型预后生物标志物和治疗靶点。
