Department of Anatomy, Faculty of Medicine, Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Chulalongkorn University, Bangkok, Thailand.
Program of Medical Science, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Oral Dis. 2021 Sep;27(6):1394-1402. doi: 10.1111/odi.13639. Epub 2020 Sep 24.
To investigate the gene expression profile of peripheral blood mononuclear cells (PBMCs) from head and neck squamous cell carcinoma (HNSCC), including oral cancer (OC) and oropharyngeal cancer (OPC) patients, and compare them with healthy controls (HC).
Transcriptomic analysis of PBMCs was performed by RNA-sequencing. The upregulated candidate genes were selected for validation by quantitative real-time polymerase chain reaction (qPCR). In addition, related plasma protein levels were determined by enzyme-linked immunosorbent assay (ELISA).
Three significantly upregulated genes, including high mobility group nucleosomal binding domain 2 (HMGN2), folate receptor gamma (FOLR3), and amphiregulin (AREG), were selected. In the first cohort, the results showed that only HMGN2 expression was significantly increased in OC patients. In the larger sample size, the overall results demonstrated that HMGN2 expression had a tendency to increase in both OC and OPC patients compared with HC. Interestingly, the plasma HMGN2 (HMG-17) protein level exhibited the same trend as that observed at the transcriptional level.
HMGN2 expression and plasma HMG-17 (HMGN2 protein) were increased in both cancer patients compared with HC. This gene may be important for further functional studies in the PBMCs of HNSCC patients.
研究头颈部鳞状细胞癌（HNSCC）患者外周血单个核细胞（PBMC）的基因表达谱，包括口腔癌（OC）和口咽癌（OPC）患者，并将其与健康对照（HC）进行比较。
通过 RNA 测序对 PBMC 的转录组进行分析。选择上调的候选基因进行定量实时聚合酶链反应（qPCR）验证。此外，通过酶联免疫吸附试验（ELISA）测定相关血浆蛋白水平。
选择了三个明显上调的基因，包括高迁移率族核小体结合域 2（HMGN2）、叶酸受体γ（FOLR3）和 Amphiregulin（AREG）。在第一个队列中，结果表明仅 OC 患者的 HMGN2 表达显著增加。在更大的样本量中，总体结果表明与 HC 相比，OC 和 OPC 患者的 HMGN2 表达均有增加的趋势。有趣的是，血浆 HMGN2（HMG-17）蛋白水平表现出与转录水平观察到的相同趋势。
与 HC 相比，癌症患者的 HMGN2 表达和血浆 HMG-17（HMGN2 蛋白）均升高。该基因可能对头颈部鳞状细胞癌患者 PBMC 的进一步功能研究很重要。
