快速重复牵伸抑制失神经诱导的肌肉纤维化。
Fast repetitive stretch suppresses denervation-induced muscle fibrosis.
机构信息
Department of Rehabilitation, Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa, Japan.
Centers of Rehabilitation, Asanogawa General Hospital, Ishikawa, Japan.
出版信息
Muscle Nerve. 2020 Dec;62(6):746-756. doi: 10.1002/mus.27059. Epub 2020 Oct 1.
BACKGROUND
We aimed to examine the influence of different speeds of stretching on denervation-induced skeletal muscle fibrosis.
METHODS
Stretching was passively applied to rat plantaris muscle denervated by sciatic nerve excision in three different cycles of 0.5, 3, or 12 cycles/min, for 20 min/d for 2 weeks.
RESULTS
Gene analysis results showed greater expression of fibrosis-related factors with fast stretching compared with non-stretched muscle. Laser Doppler blood flow analysis indicated reduced intramuscular blood flow during stretching. Histological analysis demonstrated fibrotic area decreases in 12 cycles/min stretched muscle compared with non-stretched muscle.
CONCLUSIONS
Slower stretching induced greater mRNA expression of collagen and fibroblasts and greater decrement of blood flow. Histologically, faster stretching suppressed fibrosis. These results suggest that fast repetitive stretching of denervated muscle might suppress processes of muscle fibrosis.
背景
我们旨在研究不同拉伸速度对失神经诱导的骨骼肌纤维化的影响。
方法
通过坐骨神经切除使大鼠比目鱼肌去神经，然后在 0.5、3 或 12 个/分钟三个不同的循环中以被动方式对去神经的比目鱼肌进行拉伸，每个循环拉伸 20 分钟，每天一次，持续 2 周。
结果
基因分析结果显示，与未拉伸的肌肉相比，快速拉伸肌肉中与纤维化相关的因子表达更高。激光多普勒血流分析表明，在拉伸过程中肌肉内血流量减少。组织学分析表明，与未拉伸的肌肉相比，12 个/分钟拉伸的肌肉中的纤维性区域减少。
结论
较慢的拉伸导致胶原蛋白和成纤维细胞的 mRNA 表达增加，以及血流减少更多。组织学上，更快的拉伸抑制了纤维化。这些结果表明，失神经肌肉的快速重复拉伸可能抑制肌肉纤维化的过程。
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