Gao Xiao-le, Duan Leng-Xin, Qiu Ke-Ke, Guo Man-Lin, Jiao Ye-Lin, Wang Dong-Mei
Medical School, Henan University of Science and Technology Luoyang 471023, China.
Zhongguo Zhong Yao Za Zhi. 2020 Aug;45(16):3915-3921. doi: 10.19540/j.cnki.cjcmm.20200321.401.
This study aimed to investigate the effect and possible mechanism of Bidens pilosa decoction on non-alcoholic fatty liver disease(NAFLD) induced by high fat and high glucose in mice. Bald/c mice were randomly divided into normal group, model group, metformin(200 mg·kg(-1)) treatment group, Bidens pilosa decoction(10 g·kg(-1)) treatment group, metformin and B. pilosa decoction(100 mg·kg(-1)+5 g·kg(-1)) treatment group. Except for the normal group, mice in the other four groups were fed with high-fat and high-glucose diet for 8 weeks to establish the non-alcoholic fatty liver model. After 4 weeks of treatment, blood was collected from the eyeballs, the mice were sacrificed, and relevant indicators were detected. The results showed that compared with the model group, blood lipid and blood glucose levels of each treatment group were significantly lower(P<0.05); HE staining results showed that liver pathological damage in each treatment group was significantly improved; oil red O staining results showed fat distribution in each treatment group significantly reduced(P<0.01); immunohistochemical staining showed that glucose regulated the protein expression of protein 78(GRP78) in liver tissues of each treatment group was also significantly reduced(P<0.01); Western blot results showed that endoplasmic reticulum stress signal pathway-related factors GRP78, phosphorylated-protein kinase R-like ER kinase(p-PERK), eukaryotic translation-initiation factor 2α(eIF2α), activating transcription factor 4(ATF4), C/EBP homologous protein(Chop), inositol requiring 1α(IRE1α), and cleaved-cysteinyl aspartate specific proteinase 12(cleaved-caspase-12) were significantly reduced(P<0.01). The results of the combined drug treatment group were better than those of the single drug treatment group. These results showed that B. pilosa decoction had the effect in improving non-alcoholic fatty liver, and its mechanism may be related to the down-regulation of the expression of endoplasmic reticulum stress(ERS)-related factors, and the reduction of the apoptosis of hepatocytes caused by ERS and the down-regulation of blood lipid and blood glucose levels.
本研究旨在探讨鬼针草水煎剂对高脂高糖诱导的小鼠非酒精性脂肪性肝病（NAFLD）的作用及可能机制。将BALB/c小鼠随机分为正常组、模型组、二甲双胍（200mg·kg⁻¹）治疗组、鬼针草水煎剂（10g·kg⁻¹）治疗组、二甲双胍与鬼针草水煎剂（100mg·kg⁻¹ + 5g·kg⁻¹）联合治疗组。除正常组外，其他四组小鼠给予高脂高糖饮食8周以建立非酒精性脂肪肝模型。治疗4周后，通过眼球采血，处死小鼠，并检测相关指标。结果显示，与模型组相比，各治疗组的血脂和血糖水平均显著降低（P＜0.05）；HE染色结果显示各治疗组肝脏病理损伤明显改善；油红O染色结果显示各治疗组脂肪分布显著减少（P＜0.01）；免疫组化染色显示各治疗组肝脏组织中葡萄糖调节蛋白78（GRP78）的蛋白表达也显著降低（P＜0.01）；Western blot结果显示内质网应激信号通路相关因子GRP78、磷酸化蛋白激酶R样内质网激酶（p-PERK）、真核翻译起始因子2α（eIF2α）、活化转录因子4（ATF4）、C/EBP同源蛋白（Chop）、肌醇需求酶1α（IRE1α）及裂解的半胱天冬酶12（cleaved-caspase-12）均显著降低（P＜0.01）。联合用药治疗组的结果优于单一药物治疗组。这些结果表明，鬼针草水煎剂具有改善非酒精性脂肪肝的作用，其机制可能与下调内质网应激（ERS）相关因子的表达、减少ERS引起的肝细胞凋亡以及降低血脂和血糖水平有关。
