NIBRT GlycoScience Group, National Institute for Bioprocessing, Research and Training, Blackrock, Dublin, Ireland.
CÚRAM, SFI Research Centre for Medical Devices, National University of Ireland, Galway, Ireland.
J Proteome Res. 2020 Oct 2;19(10):3890-3905. doi: 10.1021/acs.jproteome.0c00435. Epub 2020 Sep 19.
Glycosylation is crucial in cellular metabolism and survival. Of interest is the role of -linked and -linked glycans in disease states. Robust analytical methods must be defined to identify suitable glycan biomarkers and glyco-therapeutics. Fortunately, in -glycan analysis, a universal enzyme exists to deglycosylate a variety of common-core structures from proteins for analysis using mass spectrometric and fluorescence techniques. Unfortunately, for their -linked counterparts, no such enzyme exists. Furthermore, -glycan heterogeneity is vast due to the lack of a common glycan core, making analysis challenging. As such, chemical methods are used to liberate -glycans, however, often to the detriment of the glycan's structure due to "peeling" reactions. This review outlines approaches for -glycan release and downstream glycomic and glycoproteomic analysis.
糖基化在细胞代谢和存活中至关重要。有趣的是，-连接和 -连接聚糖在疾病状态中的作用。必须定义强大的分析方法来识别合适的聚糖生物标志物和糖治疗药物。幸运的是，在 -聚糖分析中，存在一种通用酶，可以将蛋白质中的各种常见核心结构糖基化，以便使用质谱和荧光技术进行分析。不幸的是，对于它们的 -连接对应物，不存在这样的酶。此外，由于缺乏共同的聚糖核心，-聚糖的异质性非常广泛，使得分析具有挑战性。因此，使用化学方法释放 -聚糖，但是，由于“剥离”反应，通常会损害聚糖的结构。本综述概述了 -聚糖释放及下游糖组学和糖蛋白质组学分析的方法。
