脉冲场凝胶电泳的沙门氏菌分子分型。

Molecular Typing of Salmonella by Pulsed-Field Gel Electrophoresis.

机构信息

Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, China.

Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China.

出版信息

Methods Mol Biol. 2021;2182:187-196. doi: 10.1007/978-1-0716-0791-6_17.

DOI:10.1007/978-1-0716-0791-6_17

PMID:32894497

Abstract

Salmonella is recognized as a major human foodborne pathogen and threat to public health world widely. It is important to carry out epidemiological investigations to determine the primary sources of bacterial contamination. Pulsed-field gel electrophoresis (PFGE) is an important method of the molecular typing, and play an important role in tracking the sources of infection and epidemic control. The PFGE is currently considered as "gold standard" of molecular typing methods for bacterial foodborne pathogen. Here, we describe the PFGE protocol to type the Salmonella from pork.

摘要

沙门氏菌被公认为主要的食源性人类病原体，也是对全球公共卫生的重大威胁。开展流行病学调查以确定细菌污染的主要来源非常重要。脉冲场凝胶电泳（PFGE）是分子分型的重要方法，在追踪感染源和疫情控制方面发挥着重要作用。PFGE 目前被认为是细菌食源性病原体分子分型方法的“金标准”。在这里，我们描述了用于对猪肉中沙门氏菌进行分型的 PFGE 方案。