Chen Zhengquan, Bai Jie, Zhang Xibin, Wang Shaojun, Chen Kaifeng, Lin Qijie, Xu Chenggang, Qu Xiaoyun, Zhang Hongxia, Liao Ming, Zhang Jianmin
National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Zoonoses, Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China.
Lab of Beef Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, PR China; New Hope Liuhe Co., Ltd., Beijing 100102, PR China.
Int J Food Microbiol. 2021 Feb 16;340:109055. doi: 10.1016/j.ijfoodmicro.2021.109055. Epub 2021 Jan 12.
This study was undertaken to investigate the prevalence, serotype distribution and antimicrobial resistance in Salmonella isolated from retail meat in Southern China, and to characterize the major mechanisms that mediate the ciprofloxacin resistance of isolates. High levels of Salmonella contamination were detected in pork (67.0%), duck (50.5%) and chicken (46.2%). Thirty different serotypes were identified among 500 detected Salmonella isolates, as well as significant differences in serotypes between different retail meat samples. Notably, 405 (80.1%) isolates exhibited multidrug resistance (MDR). Meanwhile, we also found that 74 (14.8%) Salmonella isolates were resistant to ciprofloxacin and the major mechanisms underlying this resistance were investigated. The commonest mutations in gyrA S83F (40.5%) and D87N (35.1%), and in parC was T57S (71.6%) and S80I (35.1%). Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) analysis revealed that the S. Kentucky isolates that were resistant to ciprofloxacin mostly belonged to ST198 (21/23, 91.3%) and PFGE revealed the presence of various genotypes. This study identified a diversity of Salmonella serotypes and a high prevalence of multidrug resistance (MDR) among Salmonella isolated from retail meat in Southern China, which indicates that foodborne Salmonella potentially constitutes a potential food safety risk.
本研究旨在调查中国南方零售肉类中分离出的沙门氏菌的流行情况、血清型分布及抗菌药物耐药性，并确定介导分离株对环丙沙星耐药的主要机制。在猪肉（67.0%）、鸭肉（50.5%）和鸡肉（46.2%）中检测到高水平的沙门氏菌污染。在500株检测到的沙门氏菌分离株中鉴定出30种不同的血清型，不同零售肉类样品之间的血清型也存在显著差异。值得注意的是，405株（80.1%）分离株表现出多重耐药（MDR）。同时，我们还发现74株（14.8%）沙门氏菌分离株对环丙沙星耐药，并对这种耐药的主要机制进行了研究。gyrA基因最常见的突变是S83F（40.5%）和D87N（35.1%），parC基因的突变是T57S（71.6%）和S80I（35.1%）。多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）分析显示，对环丙沙星耐药的肯塔基沙门氏菌分离株大多属于ST198（21/23，91.3%），PFGE显示存在多种基因型。本研究确定了中国南方零售肉类中分离出的沙门氏菌血清型多样，多重耐药（MDR）的流行率高，这表明食源性沙门氏菌可能构成潜在的食品安全风险。
