Li Li-Jun, Yang Guo-Hui
Clinical Medicine College of Guizhou Medical Unicersity,Guiyang 550001,China.
Intensive Care Unit,Affiliated Hospital of Guizhou Medical University,Guiyang 550001,China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2020 Aug 30;42(4):468-476. doi: 10.3881/j.issn.1000-503X.11686.
To establish an improved animal model of sepsis induced by cecal ligation and puncture(CLP). Ninety-six male Sprague-Dawley rats were randomly divided into sham operation group(=24),intubation group(=24),CLP group(=24),and CLP+intubation group(=24).The mortality rate,abdominal cavity condition,pathological changes and pathological scores of heart,lungs,liver,and kidneys of rats in each group were observed after modeling.Blood samples were obtained from the inferior vena cava for measuring the whole blood cells(WBC)and platelets(PLT)counts and analyzing serum interleukin(IL)-6,tumor necrosis factor(TNF)-α,serum troponin T(cTnT),creatine kinase-MB(CK-MB),alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),creatinine(CREA),and blood urea nitrogen(BUN)levels.Blood gas analysis of the aorta was also performed. The mortality rates 24 h after modeling were 0 in sham operation group and intubation group,20.8% in CLP group,and 54.2% in CLP+intubation group.Pathologically,swelling and inflammatory cell infiltration in the heart,lungs,liver,and kidneys were seen in the CLP+intubation group,inflammatory cell infiltration in a single organ was seen in most rats in the CLP group,and no obvious swelling and infiltration of inflammatory cells was observed in the sham-operation group and intubation group.The myocardial histopathology score,lung tissue injury pathology score,and kidney tissue injury pathology score in both the sham-operation group and the intubation group were significantly lower than those in the CLP group and the CLP+intubation group(all =0.000).TNF-α,PaO,CK-MB,cTnT,AST,TBIL,BUN,and CREA were significantly different between sham-operation group and intubation group/CLP group/CLP+intubation group and between intubation group and CLP group/CLP+intubation group(all =0.000).The pH level was significantly different between sham operation group and intubation group/CLP group,between intubation group and CLP group/CLP+intubate group(all =0.000). Although both CLP and CLP+intubation can well mimic the pathophysiological mechanism of sepsis in rats,multiple organ dysfunction occurs in the latter.Thus,CLP+intubation can establish animal models of multiple organ dysfunction caused by sepsis induced by clinically effective abdominal infection.
建立一种改良的盲肠结扎穿孔（CLP）诱导的脓毒症动物模型。将96只雄性Sprague-Dawley大鼠随机分为假手术组（n = 24）、插管组（n = 24）、CLP组（n = 24）和CLP + 插管组（n = 24）。建模后观察各组大鼠的死亡率、腹腔情况、心脏、肺、肝脏和肾脏的病理变化及病理评分。从下腔静脉采集血样，检测全血细胞（WBC）和血小板（PLT）计数，并分析血清白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α、血清肌钙蛋白T（cTnT）、肌酸激酶同工酶MB（CK-MB）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆红素（TBIL）、肌酐（CREA）和血尿素氮（BUN）水平。同时对主动脉进行血气分析。建模后24小时的死亡率，假手术组和插管组为0，CLP组为20.8%，CLP + 插管组为54.2%。病理上，CLP + 插管组心脏、肺、肝脏和肾脏出现肿胀和炎性细胞浸润，CLP组大多数大鼠单个器官出现炎性细胞浸润，假手术组和插管组未观察到明显的炎性细胞肿胀和浸润。假手术组和插管组的心肌组织病理学评分、肺组织损伤病理评分和肾组织损伤病理评分均显著低于CLP组和CLP + 插管组（均P = 0.000）。TNF-α、PaO₂、CK-MB、cTnT、AST、TBIL、BUN和CREA在假手术组与插管组/CLP组/CLP + 插管组之间以及插管组与CLP组/CLP + 插管组之间均有显著差异（均P = 0.000）。pH值在假手术组与插管组/CLP组之间、插管组与CLP组/CLP + 插管组之间均有显著差异（均P = 0.000）。虽然CLP和CLP + 插管均可很好地模拟大鼠脓毒症的病理生理机制，但后者会发生多器官功能障碍。因此，CLP + 插管可建立临床有效的腹部感染诱导的脓毒症所致多器官功能障碍的动物模型。
