Peklo M M, Liubimov A V, Printseva O Iu
Biull Eksp Biol Med. 1988 May;105(5):611-3.
The distribution of basal membrane glycoproteins, laminin and entactin, was studied immunohistochemically by peroxidase-antiperoxidase technique in different adult human organs: kidneys, liver, heart, skin, spleen and ileum. Monoclonal antibody against entactin (ELM2) reacted with all basal membranes. Monoclonal antibody against laminin (LT3.1), however, did not react with basal membranes of arterial smooth muscle cells, or with endothelial basal membranes of renal and hepatic sinusoid endothelium. Thus, LT3.1 antibody has revealed basal membrane heterogeneity by laminin. The possible immunochemical heterogeneity of basal membranes by entactin is also discussed.
采用过氧化物酶-抗过氧化物酶技术，通过免疫组织化学方法研究了层粘连蛋白和巢蛋白这两种基底膜糖蛋白在不同成人器官（肾脏、肝脏、心脏、皮肤、脾脏和回肠）中的分布情况。抗巢蛋白单克隆抗体（ELM2）可与所有基底膜发生反应。然而，抗层粘连蛋白单克隆抗体（LT3.1）不与动脉平滑肌细胞的基底膜反应，也不与肾和肝血窦内皮细胞的内皮基底膜反应。因此，LT3.1抗体揭示了层粘连蛋白导致的基底膜异质性。同时还讨论了巢蛋白导致基底膜可能存在的免疫化学异质性。
