Department of Physical Therapy, Asia University, Taichung, Taiwan, ROC.
Department of Physical Therapy and Assistive Technology, National Yang-Ming University, Taipei, Taiwan, ROC.
Brain Res. 2020 Dec 1;1748:147097. doi: 10.1016/j.brainres.2020.147097. Epub 2020 Sep 5.
Hyperbaric oxygen (HBO) has been suggested as a possible therapy for brain injury. However, the effects of HBO after transient brain ischemia are inconsistent and the underlying mechanisms are not fully known. The present study aimed to investigate the effects of repetitive HBO intervention in a transient middle cerebral artery occlusion (MCAO) animal model. Seventy-two Sprague-Dawley rats received MCAO and were randomly assigned to normal air control or HBO intervention groups. Each group was divided into 3 subgroups according to the intervention time period (7, 14, and 21 days). HBO was started 24 h post-MCAO for 1 h/day at 3.0 ATA with no-air breaks. After the final intervention, half of the rats in each subgroup were sacrificed and the right motor cortex was removed to examine levels of Akt phosphorylation and glutathione (GSH), as well as glutathione peroxidase (GPx) and reductase (GR) activity. The other half of the rats were used to examine infarct volume. At 24 h post-MCAO and the end of the final intervention, rats underwent tests to examine motor performance. We noted that 14- and 21-day HBO interventions significantly reduced infarct volume and increased Akt phosphorylation and GSH levels and GPx and GR activity. Motor performance was also significantly improved after 14- and 21-day interventions. No significant differences were observed between the controls and 7-day intervention groups. Repetitive HBO intervention starting 24 h post-MCAO and applied for at least 14 days, provided neuroprotective effects through modulating the cell survival pathway and antioxidative defense system.
高压氧（HBO）已被提议作为脑损伤的一种可能治疗方法。然而，HBO 对短暂性脑缺血后的影响并不一致，其潜在机制尚不完全清楚。本研究旨在探讨重复 HBO 干预对短暂性大脑中动脉闭塞（MCAO）动物模型的影响。72 只 Sprague-Dawley 大鼠接受 MCAO 并随机分为正常空气对照组和 HBO 干预组。每组根据干预时间（7、14 和 21 天）进一步分为 3 个亚组。MCAO 后 24 小时开始，每天 1 小时在 3.0ATA 下进行无空气中断的 HBO 治疗。最后一次干预后，每个亚组的一半大鼠被处死，取出右侧运动皮层，检测 Akt 磷酸化和谷胱甘肽（GSH）水平，以及谷胱甘肽过氧化物酶（GPx）和还原酶（GR）活性。另一半大鼠用于检测梗死体积。在 MCAO 后 24 小时和最后一次干预结束时，大鼠进行运动性能测试。我们注意到，14 天和 21 天的 HBO 干预显著降低了梗死体积，增加了 Akt 磷酸化和 GSH 水平以及 GPx 和 GR 活性。14 天和 21 天干预后运动性能也显著改善。与对照组和 7 天干预组相比，差异无统计学意义。MCAO 后 24 小时开始的重复 HBO 干预，至少持续 14 天，通过调节细胞存活途径和抗氧化防御系统提供神经保护作用。
