SYBR Green I 产生的单线态氧对液态晶相 DNA 中鸟嘌呤的损伤。

Guanine damage by singlet oxygen from SYBR Green I in liquid crystalline DNA.

机构信息

Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.

出版信息

Org Biomol Chem. 2020 Sep 23;18(36):7183-7187. doi: 10.1039/d0ob01723j.

DOI:10.1039/d0ob01723j

PMID:32897281

Abstract

It is known that double-stranded DNA (dsDNA) turns into a liquid crystalline phase by the addition of a high concentration of polymer with salt. SYBR Green I (SG) is a well-known sensitive fluorescent stain for dsDNA, and is intercalated in liquid crystalline DNA. Formation of the liquid crystalline dsDNA-SG complex has been confirmed by CD spectral measurements, fluorescence spectral measurements and confocal fluorescence microscopy. SG in dsDNA was also used as a singlet oxygen generator. We conducted photoirradiation experiments using three kinds of 42-mer oligonucleotides with SG. The amount of guanine decomposition by selective irradiation of SG was analyzed using HPLC after digestion of dsDNA in each sample solution. We found that singlet oxygen produced in liquid crystalline DNA promoted guanine damage much more efficiently than in homogeneous solution.

摘要

已知双链 DNA（dsDNA）在添加高浓度盐的聚合物后会变成液晶相。SYBR Green I（SG）是一种众所周知的用于 dsDNA 的灵敏荧光染料，可嵌入液晶 DNA 中。通过圆二色光谱测量、荧光光谱测量和共聚焦荧光显微镜证实了液晶 dsDNA-SG 配合物的形成。SG 在 dsDNA 中也被用作单线态氧发生器。我们使用三种带有 SG 的 42 聚核苷酸进行了光辐照实验。通过在每种样品溶液中的 dsDNA 消化后使用 HPLC 分析选择性辐照 SG 时鸟嘌呤的分解量。我们发现，液晶 DNA 中产生的单线态氧比均相溶液更有效地促进鸟嘌呤损伤。