Faculty of Engineering and Technology, Dong Thap University, 783 Pham Huu Lau Street, Cao Lanh City, Dong Thap Province, Viet Nam.
Center for Chemical analysis, Dong Thap University, 783 Pham Huu Lau Street, Cao Lanh City, Dong Thap Province, Viet Nam.
FEMS Microbiol Lett. 2020 Sep 25;367(18). doi: 10.1093/femsle/fnaa151.
Herbicides have been extensively used globally, resulting in severe environmental pollution. Novel butachlor-degrading Pseudomonas sp. strain But2 isolated from soil can degrade butachlor regardless of the concentration and grows without a lag phase. Specific degradation was increased at 0.01-0.1 mM, and did not change significantly at higher concentrations. During degradation, 2-chloro-N-(2,6-diethylphenyl) acetamide, 2,6-diethylaniline, and 1,3-diethylbenzene were formed, which indicated that deamination occurred. Moreover, Pseudomonas sp. strains could tolerate propanil at up to 0.8 mM. The mixed bacterial culture of Pseudomonas sp. But2 and Acinetobacter baumannii DT (a propanil-degrading bacterial strain) showed highly effective biodegradation of both butachlor and propanil in liquid media and soil. For example, under treatment with the mixed culture, the half-lives of propanil and butachlor were 1 and 5 days, respectively, whereas those for the control were 3 and 15 days. The adjuvants present in herbicides reduced degradation in liquid media, but did not influence herbicide removal from the soil. The results showed that the mixed bacteria culture is a good candidate for the removal of butachlor and propanil from contaminated soils.
除草剂被广泛应用于全球，导致了严重的环境污染。从土壤中分离到的新型丁草胺降解假单胞菌菌株 But2 可以在没有迟滞期的情况下降解丁草胺，无论浓度如何。在 0.01-0.1 mM 时，特异性降解增加，而在更高浓度时则没有显著变化。在降解过程中，形成了 2-氯-N-(2,6-二乙基苯基)乙酰胺、2,6-二乙基苯胺和 1,3-二乙基苯，这表明发生了脱氨作用。此外，假单胞菌菌株可以耐受高达 0.8 mM 的丙草胺。假单胞菌 But2 和鲍曼不动杆菌 DT（一种丙草胺降解菌）的混合细菌培养物在液体培养基和土壤中对丁草胺和丙草胺表现出高效的生物降解作用。例如，在混合培养物的处理下，丙草胺和丁草胺的半衰期分别为 1 天和 5 天，而对照的半衰期分别为 3 天和 15 天。除草剂中的助剂降低了液体培养基中的降解，但不会影响土壤中除草剂的去除。结果表明，混合细菌培养物是去除污染土壤中丁草胺和丙草胺的良好候选物。
