Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, China.
School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, 750004, China.
J Mol Histol. 2020 Dec;51(6):629-637. doi: 10.1007/s10735-020-09909-1. Epub 2020 Sep 8.
Lycium barbarum polysaccharide (LBP) is an alkaloid extracted from lycium barbarum. LBP is the active component of lycium barbarum used to treat hypertension, atherosclerosis and other cardiovascular diseases in Chinese traditional medicine. However, the underlying cellular and molecular mechanisms of LBP- mediated activity in vascular disease remain poorly understood. In the present study, we showed the protective effect of LBP in vascular smooth muscle cells. Our results indicate that LBP significantly reduces the proliferation of VSMCs caused by Homocysteine (Hcy) and inhibits the phenotypic transformation of VSMCs caused by Hcy, from contractile to synthetic. LBP inhibited the protein expression of PI3K and Akt caused by Hcy, and increased the expression of miR-145. The results indicate that LBP exhibits substantial therapeutic potential for the treatment of Hcy-induced VSMCs proliferation and phenotypic transformation through inhibition of PI3K/Akt signaling pathway.
枸杞多糖（LBP）是从枸杞中提取的一种生物碱。LBP 是枸杞的活性成分，用于治疗中医中的高血压、动脉粥样硬化和其他心血管疾病。然而，LBP 介导的血管疾病活性的潜在细胞和分子机制仍知之甚少。在本研究中，我们展示了 LBP 在血管平滑肌细胞中的保护作用。我们的结果表明，LBP 可显著减轻同型半胱氨酸（Hcy）引起的 VSMCs 增殖，并抑制 Hcy 引起的 VSMCs 表型转化，使收缩型向合成型转化。LBP 抑制了 Hcy 引起的 PI3K 和 Akt 蛋白表达，并增加了 miR-145 的表达。结果表明，LBP 通过抑制 PI3K/Akt 信号通路，对 Hcy 诱导的 VSMCs 增殖和表型转化具有显著的治疗潜力。
