Ziarno Małgorzata, Bryś Joanna, Parzyszek Mateusz, Veber Anna
Division of Milk Technology, Department of Food Technology and Assessment, Institute of Food Science, Warsaw University of Life Sciences-SGGW (WULS-SGGW), 02-787 Warsaw, Poland.
Department of Chemistry, Institute of Food Science, Warsaw University of Life Sciences-SGGW (WULS-SGGW), 02-787 Warsaw, Poland.
Microorganisms. 2020 Sep 3;8(9):1348. doi: 10.3390/microorganisms8091348.
Biological processes of legumes may change their nutritional value of lipids, but there is no research on the fatty acid profile and their position distribution in fermented beverages obtained from germinated bean seeds. The present study aimed to determine the effect of fermentation by strains on the fatty acid profile and their positional distribution in triacylglycerols of beverage obtained from germinated bean "Piękny Jaś Karłowy" () fermented by strains. The population of lactobacilli (the pour plate method), pH, the fatty acid profile (gas chromatograph with a flame ionization detector), and the positional distribution of fatty acids in triacylglycerols (GC-FID) were determined before and after the fermentation of received beverages. The fermentation of beverages did not change the lactobacilli population (over 7 log CFU/g), but changed pH (to 4.7-3.7 or 5.8-5.6), fatty acid profile, and the positional distribution of fatty acids were observed. The fermentation process contributed to an increase in the share of palmitic, stearic, and oleic acids in the fatty acid profile compared to that in raw bean seeds. The fermentation processes changed the share of individual acids in positions sn-1 and sn-3 depending on strain used. Compared to non-fermented beverages, in most fermented beverages, a lower share of palmitic and stearic acids, as well as a higher share of oleic acid in the sn-2 were observed.
豆类的生物过程可能会改变其脂质的营养价值,但关于发芽豆种子发酵饮料中的脂肪酸谱及其位置分布尚无研究。本研究旨在确定菌株发酵对“Piękny Jaś Karłowy”发芽豆发酵饮料中三酰甘油的脂肪酸谱及其位置分布的影响。在接收到的饮料发酵前后,测定了乳酸菌数量(倾注平板法)、pH值、脂肪酸谱(带火焰离子化检测器的气相色谱仪)以及三酰甘油中脂肪酸的位置分布(气相色谱-火焰离子化检测器)。饮料发酵并未改变乳酸菌数量(超过7 log CFU/g),但改变了pH值(至4.7 - 3.7或5.8 - 5.6),观察到脂肪酸谱以及脂肪酸的位置分布发生了变化。与未发酵的豆种子相比,发酵过程使脂肪酸谱中棕榈酸、硬脂酸和油酸的占比增加。发酵过程根据所使用的菌株改变了sn-1和sn-3位置上各脂肪酸的占比。与未发酵饮料相比,在大多数发酵饮料中,sn-2位的棕榈酸和硬脂酸占比更低,油酸占比更高。