抑郁小鼠海马组织和血液中 microRNA-17 的表达下调可上调 PAI-1 mRNA 和蛋白的表达。
Decreased expression of microRNA-17 in hippocampal tissues and blood from mice with depression up-regulates the expression of PAI-1 mRNA and protein.
机构信息
Department of Psychology, The Second Children and Women's Healthcare of Jinan City, Jinan, China.
Department of Gynecology, The Second Children and Women's Healthcare of Jinan City, Jinan, China.
出版信息
Braz J Med Biol Res. 2020;53(10):e8826. doi: 10.1590/1414-431X20208826. Epub 2020 Sep 7.
This study determined the expression of plasminogen activator inhibitor-1 (PAI-1) and microRNA (miR)-17 in a mouse depression model. Forty male mice were divided evenly into control and depression groups. A chronic unpredictable mild stress (CUMS) model was constructed. qRT-PCR was used to determine the expression of PAI-1 mRNA and miR-17. Western blotting and ELISA were used to determine expression of PAI-1 protein. Dual luciferase reporter assay was carried out to identify direct interaction between miR-17 and PAI-1 mRNA. The mice with depression had elevated PAI-1 mRNA and protein in hippocampal tissues and blood. Expression of miR-17 was decreased in hippocampal tissues and blood from mice with depression. miR-17 bound with the 3'-UTR of PAI-1 mRNA to regulate its expression. This study demonstrated that miR-17 expression in hippocampal tissues and blood from mice with depression was decreased while expression of PAI-1 mRNA and protein was up-regulated. miR-17 participated in depression in mice by regulating PAI-1.
本研究旨在确定纤溶酶原激活物抑制剂-1（PAI-1）和 microRNA（miR）-17 在小鼠抑郁模型中的表达。将 40 只雄性小鼠平均分为对照组和抑郁组。构建慢性不可预测轻度应激（CUMS）模型。采用 qRT-PCR 检测 PAI-1mRNA 和 miR-17 的表达。采用 Western blot 和 ELISA 检测 PAI-1 蛋白的表达。通过双荧光素酶报告实验鉴定 miR-17 与 PAI-1mRNA 之间的直接相互作用。患有抑郁症的小鼠海马组织和血液中的 PAI-1mRNA 和蛋白表达升高。患有抑郁症的小鼠海马组织和血液中的 miR-17 表达降低。miR-17 与 PAI-1mRNA 的 3'-UTR 结合，调节其表达。本研究表明，患有抑郁症的小鼠海马组织和血液中的 miR-17 表达降低，而 PAI-1mRNA 和蛋白的表达上调。miR-17 通过调节 PAI-1 参与小鼠的抑郁。
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