Suppr超能文献

bZIP 二聚体 CREB1、ATF2、Zta、ATF3|cJun 和 cFos|cJun 优先与包含 5-甲酰胞嘧啶和 5-羧基胞嘧啶的某些双链 DNA 序列结合。

bZIP Dimers CREB1, ATF2, Zta, ATF3|cJun, and cFos|cJun Prefer to Bind to Some Double-Stranded DNA Sequences Containing 5-Formylcytosine and 5-Carboxylcytosine.

出版信息

Biochemistry. 2020 Sep 29;59(38):3529-3540. doi: 10.1021/acs.biochem.0c00475. Epub 2020 Sep 20.

Abstract

In mammalian cells, 5-methylcytosine (5mC) occurs in genomic double-stranded DNA (dsDNA) and is enzymatically oxidized to 5-hydroxymethylcytosine (5hmC), then to 5-formylcytosine (5fC), and finally to 5-carboxylcytosine (5caC). These cytosine modifications are enriched in regulatory regions of the genome. The effect of these oxidative products on five bZIP dimers (CREB1, ATF2, Zta, ATF3|cJun, and cFos|cJun) binding to five types of dsDNA was measured using protein binding microarrays. The five dsDNAs contain either cytosine in both DNA strands or cytosine in one strand and either 5mC, 5hmC, 5fC, or 5caC in the second strand. Some sequences containing the CEBP half-site GCAA are bound more strongly by all five bZIP domains when dsDNA contains 5mC, 5hmC, or 5fC. dsDNA containing 5caC in some TRE (AP-1)-like sequences, e.g., TGACTAA, is better bound by Zta, ATF3|cJun, and cFos|cJun.

摘要

在哺乳动物细胞中,5-甲基胞嘧啶(5mC)存在于基因组双链 DNA(dsDNA)中,并被酶氧化为 5-羟甲基胞嘧啶(5hmC),然后是 5-甲酰基胞嘧啶(5fC),最后是 5-羧基胞嘧啶(5caC)。这些胞嘧啶修饰在基因组的调控区域中富集。使用蛋白质结合微阵列测量了这些氧化产物对五个 bZIP 二聚体(CREB1、ATF2、Zta、ATF3|cJun 和 cFos|cJun)与五种类型 dsDNA 结合的影响。五种 dsDNA 要么两条 DNA 链上都有胞嘧啶,要么一条 DNA 链上有胞嘧啶,另一条 DNA 链上有 5mC、5hmC、5fC 或 5caC。一些包含 CEBP 半位点 GCAA 的序列,当 dsDNA 含有 5mC、5hmC 或 5fC 时,所有五个 bZIP 结构域的结合更紧密。在一些 TRE(AP-1)样序列(例如 TGACTAA)中,dsDNA 中含有 5caC,Zta、ATF3|cJun 和 cFos|cJun 的结合更好。

相似文献

2
The Epstein-Barr Virus B-ZIP Protein Zta Recognizes Specific DNA Sequences Containing 5-Methylcytosine and 5-Hydroxymethylcytosine.
Biochemistry. 2017 Nov 28;56(47):6200-6210. doi: 10.1021/acs.biochem.7b00741. Epub 2017 Nov 10.
3
Replacing C189 in the bZIP domain of Zta with S, T, V, or A changes DNA binding specificity to four types of double-stranded DNA.
Biochem Biophys Res Commun. 2018 Jul 2;501(4):905-912. doi: 10.1016/j.bbrc.2018.05.080. Epub 2018 May 18.
6
7
Weakened N3 Hydrogen Bonding by 5-Formylcytosine and 5-Carboxylcytosine Reduces Their Base-Pairing Stability.
ACS Chem Biol. 2016 Feb 19;11(2):470-7. doi: 10.1021/acschembio.5b00762. Epub 2015 Dec 17.
8
Altering the Double-Stranded DNA Specificity of the bZIP Domain of Zta with Site-Directed Mutagenesis at N182.
ACS Omega. 2021 Dec 28;7(1):129-139. doi: 10.1021/acsomega.1c04148. eCollection 2022 Jan 11.
9
Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA.
Nature. 2014 Feb 20;506(7488):391-5. doi: 10.1038/nature12905. Epub 2013 Dec 25.
10
5-Methylcytosine (5mC) and 5-Hydroxymethylcytosine (5hmC) Enhance the DNA Binding of CREB1 to the C/EBP Half-Site Tetranucleotide GCAA.
Biochemistry. 2016 Dec 13;55(49):6940-6948. doi: 10.1021/acs.biochem.6b00796. Epub 2016 Nov 29.

引用本文的文献

1
Altering the Double-Stranded DNA Specificity of the bZIP Domain of Zta with Site-Directed Mutagenesis at N182.
ACS Omega. 2021 Dec 28;7(1):129-139. doi: 10.1021/acsomega.1c04148. eCollection 2022 Jan 11.

本文引用的文献

1
Toward a Mechanistic Understanding of DNA Methylation Readout by Transcription Factors.
J Mol Biol. 2020 Mar 13;432(6):1801-1815. doi: 10.1016/j.jmb.2019.10.021. Epub 2019 Nov 2.
2
TET-Catalyzed 5-Carboxylcytosine Promotes CTCF Binding to Suboptimal Sequences Genome-wide.
iScience. 2019 Sep 27;19:326-339. doi: 10.1016/j.isci.2019.07.041. Epub 2019 Jul 30.
3
Protein Interactions at Oxidized 5-Methylcytosine Bases.
J Mol Biol. 2020 Mar 13;432(6):1718-1730. doi: 10.1016/j.jmb.2019.07.039. Epub 2019 Aug 8.
4
Structural basis for preferential binding of human TCF4 to DNA containing 5-carboxylcytosine.
Nucleic Acids Res. 2019 Sep 19;47(16):8375-8387. doi: 10.1093/nar/gkz381.
5
The bZIP mutant CEBPB (V285A) has sequence specific DNA binding propensities similar to CREB1.
Biochim Biophys Acta Gene Regul Mech. 2019 Apr;1862(4):486-492. doi: 10.1016/j.bbagrm.2019.02.002. Epub 2019 Feb 27.
6
Replacing C189 in the bZIP domain of Zta with S, T, V, or A changes DNA binding specificity to four types of double-stranded DNA.
Biochem Biophys Res Commun. 2018 Jul 2;501(4):905-912. doi: 10.1016/j.bbrc.2018.05.080. Epub 2018 May 18.
7
The Epstein-Barr Virus B-ZIP Protein Zta Recognizes Specific DNA Sequences Containing 5-Methylcytosine and 5-Hydroxymethylcytosine.
Biochemistry. 2017 Nov 28;56(47):6200-6210. doi: 10.1021/acs.biochem.7b00741. Epub 2017 Nov 10.
8
TET-mediated active DNA demethylation: mechanism, function and beyond.
Nat Rev Genet. 2017 Sep;18(9):517-534. doi: 10.1038/nrg.2017.33. Epub 2017 May 30.
9

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验