Neurovirology Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Microbiol Immunol. 2020 Dec;64(12):835-839. doi: 10.1111/1348-0421.12843. Epub 2020 Nov 6.
After eradication and containment of wild poliovirus (PV) and cessation of oral polio vaccinations, it is critical to minimize the risk of reintroducing PV into PV-free communities via facilities that handle the virus. The potential risk of unintentional PV propagation through unidentified contaminated materials is a serious issue. This study reports the generation of HeLa and RD-A cells deficient in functional CD155 gene (∆PVR cells); these cells are not susceptible to PV but remain susceptible to other picornaviruses. These ∆PVR cells will minimize the risk of unintentional transmission of PV and support performing the experiments more safely.
在消灭和控制野生脊髓灰质炎病毒（PV）并停止口服脊髓灰质炎疫苗接种后，通过处理病毒的设施，将 PV 重新引入无 PV 社区的风险最小化至关重要。通过未识别的受污染材料意外传播 PV 的潜在风险是一个严重的问题。本研究报告了产生缺乏功能性 CD155 基因（∆PVR 细胞）的 HeLa 和 RD-A 细胞；这些细胞不易感染 PV，但仍易感染其他小核糖核酸病毒。这些 ∆PVR 细胞将最大限度地降低无意传播 PV 的风险，并支持更安全地进行实验。
