Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-9640, Japan.
Department of Virology 3 and WHO Global Specialized Laboratory for Measles and Rubella, National Institute of Infectious Diseases, 4-7-1, Gakuen, Musashimurayama, Tokyo, 208-0011, Japan.
Sci Rep. 2021 Mar 24;11(1):6746. doi: 10.1038/s41598-021-86050-3.
Polio or poliomyelitis is a disabling and life-threatening disease caused by poliovirus (PV). As a consequence of global polio vaccination efforts, wild PV serotypes 2 and 3 have been eradicated around the world, and wild PV serotype 1-transmitted cases have been largely eliminated except for limited regions. However, vaccine-derived PV, pathogenically reverted live PV vaccine strains, has become a serious issue. For the global eradication of polio, the World Health Organization is conducting the third edition of the Global Action Plan, which is requesting stringent control of potentially PV-infected materials. To facilitate the mission, we generated a PV-nonsusceptible Vero cell subline, which may serve as an ideal replacement of standard Vero cells to isolate emerging/re-emerging viruses without the risk of generating PV-infected materials.
脊灰病毒（PV）可引起小儿麻痹症，即脊髓灰质炎，该病具有致残性且能危及生命。得益于全球范围内的脊灰疫苗接种工作，除了在有限地区外，全球已经基本消灭了野生型 2 型和 3 型脊灰病毒，并且野生型 1 型脊灰病毒的传播病例也大为减少。然而，疫苗衍生脊灰病毒和具有潜在致病性的返祖活疫苗株已成为一个严重问题。为了实现全球消灭脊灰的目标，世界卫生组织正在实施第三个全球行动计划，要求严格控制可能受到脊灰病毒污染的材料。为了助力该行动计划的实施，我们构建了一株对脊灰病毒不敏感的 Vero 细胞亚系，它可以替代标准的 Vero 细胞，用于分离新出现/再现的病毒，而不会产生脊灰病毒污染材料的风险。
