Ueno T, Yasuura S, Watanabe S, Hirose M, Sekine T, Namihisa T
Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan.
J Histochem Cytochem. 1988 Jul;36(7):803-6. doi: 10.1177/36.7.3290335.
It has been established that the liver cell possesses its own myosin which resembles other non-muscle myosins in subunit composition and in its dependence of actin-activated Mg2+-ATPase activity on light chain phosphorylation (Ueno T, Sekine T: Biochem Int, 1987;15:1205). We have raised a specific antibody against rabbit liver cell myosin. Immunoblot analysis has shown that the purified antibody reacts only with the heavy chain of liver cell myosin. The antibody did not react with rabbit skeletal muscle myosin or with smooth muscle myosin extracted from rabbit intestinal wall. Cryostat liver sections analyzed by indirect immunofluorescence microscopy showed a characteristic polygonal staining pattern, indicating that myosin is concentrated close to the plasma membrane, particularly in the region of bile canaliculi. Myosin therefore appears to be localized in the area where actin filaments are also abundant.
已经确定肝细胞拥有自身的肌球蛋白，其在亚基组成以及肌动蛋白激活的Mg2 + -ATP酶活性对轻链磷酸化的依赖性方面类似于其他非肌肉肌球蛋白（上野T，关根T：生物化学国际，1987；15：1205）。我们已经制备了针对兔肝细胞肌球蛋白的特异性抗体。免疫印迹分析表明，纯化的抗体仅与肝细胞肌球蛋白的重链反应。该抗体不与兔骨骼肌肌球蛋白或从兔肠壁提取的平滑肌肌球蛋白反应。通过间接免疫荧光显微镜分析的低温恒温器肝脏切片显示出特征性的多边形染色模式，表明肌球蛋白集中在靠近质膜的区域，特别是在胆小管区域。因此，肌球蛋白似乎定位于肌动蛋白丝也丰富的区域。
