Oyaizu N, Yasumizu R, Miyama-Inaba M, Nomura S, Yoshida H, Miyawaki S, Shibata Y, Mitsuoka S, Yasunaga K, Morii S
Department of Pathology, Kansai Medical University, Osaka, Japan.
J Exp Med. 1988 Jun 1;167(6):2017-22. doi: 10.1084/jem.167.6.2017.
A decrease in thrombocyte count was observed in (NZW x BXSB)F1 (W/B F1) mice at the age of greater than 5 mo, whereas megakaryocyte counts were found to increase in such mice. FACS analyses revealed the presence of both platelet-associated antibodies (PAA) and circulating antiplatelet antibodies. There is a correlation between the presence of these antibodies and the degree of thrombocytopenia. The transplantation of normal bone marrow cells from BALB/c nu/nu mice to W/B F1 mice was found to have preventative and curative effects on thrombocytopenia; the mice showed normal platelet counts and no evidence of circulating antiplatelet antibodies. These results indicate that thrombocytopenia in W/B F1 mice is due to the presence of antibodies to platelets. We therefore think that W/B F1 mice serve as a useful animal model of idiopathic thrombocytopenic purpura (ITP) not only for elucidating the mechanism of the development of antiplatelet antibodies, but also for characterizing autoantibodies to platelets.
在大于5月龄的（新西兰白兔×BXSB）F1（W/B F1）小鼠中观察到血小板计数下降，而此类小鼠的巨核细胞计数增加。流式细胞术分析显示存在血小板相关抗体（PAA）和循环抗血小板抗体。这些抗体的存在与血小板减少程度之间存在相关性。将BALB/c nu/nu小鼠的正常骨髓细胞移植到W/B F1小鼠中，发现对血小板减少症有预防和治疗作用；小鼠血小板计数正常，且无循环抗血小板抗体的迹象。这些结果表明，W/B F1小鼠的血小板减少症是由于存在抗血小板抗体。因此，我们认为W/B F1小鼠不仅是阐明抗血小板抗体产生机制的有用动物模型，也是表征抗血小板自身抗体的有用动物模型。
