Jin Seong Eun, Shin Hyeun-Kyoo, Ha Hyekyung
Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
Integr Med Res. 2021 Mar;10(1):100466. doi: 10.1016/j.imr.2020.100466. Epub 2020 Jul 1.
Acetaminophen (-acetyl--aminophenol, APAP) is a safe and effective analgesic at therapeutic dosage. However, APAP overdose is a major cause of acute liver injury. (GMSYS; in Chinese, in Japanese), a traditional herbal formula, is used to treat phlegm and cough in Korea. The purpose of this study was to investigate the hepatoprotective effect of GMSYS against APAP-induced liver injury and .
We evaluated the effect of GMSYS on APAP-induced hepatotoxicity by measuring cell viability in murine BNL CL.2 liver cells. Additionally, BALB/c mice were orally administered with GMSYS once daily for 7 days. Eighteen hours after the last administration, mice were intraperitoneally injected with 200 mg/kg APAP. Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, hepatic antioxidants, and histological changes were examined.
Pretreatment with GMSYS attenuated the decrease in cell viability induced by APAP in BNL CL.2 cells. In mice, pre-administration with GMSYS alleviated APAP-induced hepatotoxicity by decreasing plasma ALT and AST activities and hepatic malondialdehyde, and by increasing the total glutathione (GSH)/reduced GSH ratio and the activities of several antioxidants such as superoxide dismutase, catalase, GSH peroxidase, GSH reductase, GSH-S-transferase, and heme oxygenase-1.
GMSYS has a protective effect against APAP-induced acute liver injury by decreasing plasma transaminases and increasing antioxidants. GMSYS may be an effective candidate for the prevention of acute liver injury.
对乙酰氨基酚（-乙酰--氨基酚，APAP）在治疗剂量下是一种安全有效的镇痛药。然而，APAP过量是急性肝损伤的主要原因。（GMSYS；中文、日文）是一种传统草药配方，在韩国用于治疗痰和咳嗽。本研究的目的是研究GMSYS对APAP诱导的肝损伤的肝保护作用以及。
我们通过测量小鼠BNL CL.2肝细胞的细胞活力来评估GMSYS对APAP诱导的肝毒性的影响。此外，将BALB/c小鼠每天口服一次GMSYS，持续7天。在最后一次给药18小时后，小鼠腹腔注射200 mg/kg APAP。检测血浆丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平、肝脏抗氧化剂和组织学变化。
GMSYS预处理减轻了APAP诱导的BNL CL.2细胞活力下降。在小鼠中，预先给予GMSYS可通过降低血浆ALT和AST活性以及肝脏丙二醛，并通过增加总谷胱甘肽（GSH）/还原型GSH比值以及几种抗氧化剂如超氧化物歧化酶、过氧化氢酶、GSH过氧化物酶、GSH还原酶、GSH-S-转移酶和血红素加氧酶-1的活性来减轻APAP诱导的肝毒性。
GMSYS通过降低血浆转氨酶和增加抗氧化剂对APAP诱导的急性肝损伤具有保护作用。GMSYS可能是预防急性肝损伤的有效候选药物。
