小鼠中的狂犬病病毒感染通过表观遗传修饰上调。
Rabies virus infection in mice up-regulates via epigenetic modifications.
作者信息
Ojedapo Comfort E, Muhammad Aliyu, Kia Grace S N, Abarshi Musa M, Abdulazeez Maryam, Atawodi Joy Cecilia, Kwaga Jacob K P
机构信息
Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State Nigeria.
Africa Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University Centre, Zaria, Nigeria.
出版信息
Virusdisease. 2020 Sep;31(3):388-394. doi: 10.1007/s13337-020-00588-w. Epub 2020 May 12.
Rabies virus infection is an endemic disease which remains central to public health issues. The presence of epigenetics associated with the over-expression of in mice brain infected with rabies virus was investigated for the first time. A significant increase ( < 0.05) in mRNA level of as the disease progressed was observed. The percentage of methylated region was significantly ( < 0.05) higher in infected tissues relative to uninfected. DNA methyltransferase (DNMT) and histone acetylase (HAT) activities were also significantly ( < 0.05) higher in most infected brain tissues. HAT had a relatively higher proportion than DNMT when compared to the normal. Paradoxically, it can be inferred that the rabies virus uses epigenetic mechanisms as a means of manipulating host genes, as there was an increase in global DNMT and HAT activities with concomitant increase in promoter methylation and expression.
狂犬病病毒感染是一种地方病，仍然是公共卫生问题的核心。首次对感染狂犬病病毒的小鼠大脑中与[未提及具体基因]过度表达相关的表观遗传学现象进行了研究。随着疾病进展，观察到[未提及具体基因]的mRNA水平显著升高（P<0.05）。相对于未感染组织，感染组织中甲基化区域的百分比显著更高（P<0.05）。在大多数感染的脑组织中，DNA甲基转移酶（DNMT）和组蛋白乙酰转移酶（HAT）的活性也显著更高（P<0.05）。与正常情况相比，HAT的比例相对高于DNMT。矛盾的是，可以推断狂犬病病毒利用表观遗传机制作为操纵宿主基因的一种手段，因为全局DNMT和HAT活性增加，同时[未提及具体基因]启动子甲基化和表达也增加。
Zotero 插件