Li Busu, Li Li, Wang Wei, Meng Jie, Xu Fei, Wu Fucun, Zhang Guofan
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China.
ACS Omega. 2020 Aug 19;5(34):21355-21363. doi: 10.1021/acsomega.0c01325. eCollection 2020 Sep 1.
Free fatty acid receptor 4 (FFAR4) has various physiological functions, including energy regulation and immunological homeostasis. We examined the only FFAR4 homologue in the Pacific oyster (), which functions as a sensor of long-chain fatty acids. is 1098 bp long and contains a seven-transmembrane G protein-coupled receptor domain. expression was high in the hepatopancreas, but it was downregulated after fasting, indicating that it plays an essential role in food digestion. Lipopolysaccharide stimulation downregulated level, probably as an immune response of the animal. Reduced glycogen level alongside decreased insulin receptor, insulin receptor substrate, and glycogen synthase transcription levels after knockdown revealed that was involved in the regulation of fatty acid and glycogen levels via the insulin pathway. Accordingly, this is the first study on an invertebrate FFAR and provides new insights into the role of this receptor in immune response and nutritional control.
游离脂肪酸受体4（FFAR4）具有多种生理功能，包括能量调节和免疫稳态。我们研究了太平洋牡蛎中唯一的FFAR4同源物，它作为长链脂肪酸的传感器发挥作用。该同源物长度为1098 bp，包含一个七跨膜G蛋白偶联受体结构域。其在肝胰腺中表达较高，但禁食后表达下调，表明它在食物消化中起重要作用。脂多糖刺激会下调其水平，这可能是动物的一种免疫反应。敲低该同源物后，糖原水平降低，同时胰岛素受体、胰岛素受体底物和糖原合酶转录水平下降，这表明该同源物通过胰岛素途径参与脂肪酸和糖原水平的调节。因此，这是关于无脊椎动物FFAR的首次研究，并为该受体在免疫反应和营养控制中的作用提供了新的见解。
