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太平洋牡蛎(Crassostrea gigas)摄入小微型塑料(<100μm)的证据:一种使用微傅里叶变换红外光谱法(Micro-FTIR)进行提取、纯化和分析的新方法。

Evidence of small microplastics (<100 μm) ingestion by Pacific oysters (Crassostrea gigas): A novel method of extraction, purification, and analysis using Micro-FTIR.

机构信息

Institute of Polar Sciences, CNR-ISP, Campus Scientifico - Ca' Foscari University of Venice, Via Torino, 155, 30172 Venezia-Mestre, Italy; Department of Environmental Sciences, Informatics and Statistics, Ca' Foscari University of Venice, Via Torino, 155, 30172 Venezia-Mestre, Italy.

Department of Environmental Sciences, Informatics and Statistics, Ca' Foscari University of Venice, Via Torino, 155, 30172 Venezia-Mestre, Italy.

出版信息

Mar Pollut Bull. 2020 Nov;160:111606. doi: 10.1016/j.marpolbul.2020.111606. Epub 2020 Sep 6.

DOI:10.1016/j.marpolbul.2020.111606
PMID:32905909
Abstract

Microplastics (MPs) are present in fresh, brackish, or marine waters. Micro- and macroinvertebrates can mistake MPs or small microplastics (SMPs, <100 μm) to be food particles and easily ingest them according to the size of their mouthparts. SMPs may then block the passage of food through the intestinal tract (i.e. hepatopancreas), accumulate within the organism, and enter the food web. Pacific oysters (Crassostrea gigas) are allochthonous filter-feeding bivalve mollusks, which have been introduced in coastal seas around the world in both natural banks and farms. Considering their economic and ecological value, these bivalves have been chosen as a model to study the ingestion of SMPs. A novel method for the extraction and purification of SMPs in bivalves was developed. Quantification and simultaneous polymer identification of SMPs using Micro-FTIR (Fourier Transform Infrared Spectroscopy) were performed, with a limit of detection for the particle size of 5 μm.

摘要

微塑料(MPs)存在于淡水、半咸水或海水中。微小型无脊椎动物可能会将 MPs 或小微型塑料(SMPs,<100μm)误认为食物颗粒,并根据其口器的大小轻易将其摄入体内。SMPs 可能会阻塞消化道(即肝胰腺)的食物通过,在体内积累,并进入食物网。太平洋牡蛎(Crassostrea gigas)是一种外来滤食性双壳贝类,已在世界各地的天然和人工养殖的沿海海域引入。鉴于其经济和生态价值,这些双壳贝类已被选为研究 SMPs 摄入的模型。本文开发了一种从双壳贝类中提取和纯化 SMPs 的新方法。使用 Micro-FTIR(傅里叶变换红外光谱)对 SMPs 进行定量和聚合物同时识别,检测到的颗粒尺寸下限为 5μm。

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