State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China.
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China.
Ecotoxicol Environ Saf. 2021 Jan 1;207:111251. doi: 10.1016/j.ecoenv.2020.111251. Epub 2020 Sep 6.
A mildly one-pot method is developed for the synthesis of quasi-networked AuPtCo ternary alloy nanoparticles (TANPs) at room temperature through the co-reduction of AuCl, PtCl and Co with hydrazine hydrate. Characterizations of XRD, XPS, HRTEM, EDS and SAED successfully reveal the crystal structure, composition, valence and morphology of AuPtCo TANPs, respectively. The glassy carbon electrode (GCE) modified by AuPtCo TANPs with good dispersion and multi-density surface defects occupies the optimal electrochemical active surface area (ECSA). After the coated poly-L-cysteine (P-L-Cys) film on the AuPtCo/GCE surface, the morphology, element mapping and surface roughness of the P-L-Cys/AuPtCo/GCE are investigated via FESEM and AFM to verify continuous electrode modification processes. The electrochemical behaviors of the composite electrode for hydroquinone (HQ) are evaluated by cyclic voltammetry (CV) with interfacial properties of adsorption and diffusion. Differential pulse voltammetry (DPV) for HQ electrochemical sensing at 0.10 V (vs. SCE) exhibits two linear response ranges from 0.1 to 30 and 30-200 μM, respectively. A low detection limit (S/N = 3) of 0.045 μM is obtained with a sensitivity of 4.247 μA μM·cm. The resulting P-L-Cys/AuPtCo/GCE also presents ascendant selectivity, repeatability, reproducibility and stability. In addition, the established method is applied to the assessment of the HQ level in real water samples (mineral water, tap water and lake water) with the satisfactory results of spiked recoveries. The sensor may become a promising tool for the trace analysis of the electroactive substance in food or environmental samples.
一种温和的一锅法在室温下合成准网络状的 AuPtCo 三元合金纳米粒子（TANPs），通过水合肼同时还原 AuCl、PtCl 和 Co。XRD、XPS、HRTEM、EDS 和 SAED 的特征分别成功揭示了 AuPtCo TANPs 的晶体结构、组成、价态和形态。具有良好分散性和多密度表面缺陷的 AuPtCo TANPs 修饰的玻碳电极（GCE）占据了最佳的电化学活性表面积（ECSA）。在 AuPtCo/GCE 表面涂覆聚-L-半胱氨酸（P-L-Cys）膜后，通过 FESEM 和 AFM 研究了 P-L-Cys/AuPtCo/GCE 的形貌、元素映射和表面粗糙度，以验证连续的电极修饰过程。通过循环伏安法（CV）评估复合电极对氢醌（HQ）的电化学行为，研究了界面吸附和扩散特性。在 0.10 V（相对于 SCE）下，差分脉冲伏安法（DPV）用于 HQ 电化学传感，显示出两个线性响应范围，分别为 0.1-30 和 30-200 μM。检测限（S/N = 3）低至 0.045 μM，灵敏度为 4.247 μA μM·cm。所得的 P-L-Cys/AuPtCo/GCE 还表现出上升的选择性、重复性、重现性和稳定性。此外，该方法还应用于实际水样（矿泉水、自来水和湖水）中 HQ 水平的评估，加标回收率令人满意。该传感器有望成为食品或环境样品中电活性物质痕量分析的一种有前途的工具。
