Direct visualization of phase transition dynamics in binary supported phospholipid bilayers using imaging ellipsometry.
作者信息
Szmodis Alan W, Blanchette Craig D, Levchenko Andrey A, Navrotsky Alexandra, Longo Marjorie L, Orme Christine A, Parikh Atul N
机构信息
Dept. of Applied Science, University of California, Davis, CA USA.
Biophysical and Interfacial Science Group, Chemistry and Material Science, Lawrence Livermore National Laboratory, Livermore, CA, USA.
出版信息
Soft Matter. 2008 May 14;4(6):1161-1164. doi: 10.1039/b801390j.
Via imaging ellipsometry, we study the phase transition dynamics induced by selective gelation of one component in a binary supported phopholipid bilayer. We find the modulation of two attendant morphological features: emergence of extended defect chains due to a net change in the molecular areas and fractal-like domains suggesting weak line tension. A time-lapse analysis of the ellipsometric images reveals the cluster size of 4-20 molecules undergoing gelation indicating weak cooperativity. These results demonstrate the use of ellipsometry for in situ, label-free, non-contact, and large-area imaging of dynamics in interfacial films.
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