Patel Priyesh, Irvine Scott, McEwan Jean R, Jayasinghe Suwan N
Department of Mechanical Engineering, University College London, Torrington Place, London, United KingdomWC1E 7JE.
The Royal Free and University College London Medical School, The Rayne Institute, 5 University Street, London, United KingdomWC1E 6JJ and Molecular Immunology Unit, Institute of Child Health, University College London, Guilford Street, London, United KingdomWC1N 1EH.
Soft Matter. 2008 May 14;4(6):1219-1229. doi: 10.1039/b718866h.
Electrosprays and electrospinning were recently pioneered for directly handling living cells. These recent discoveries are now widely referred to as "bio-electrosprays" and "cell electrospinning", which have been demonstrated as having tremendous applicability to the life sciences in regenerative and therapeutic medicine. In the current work, we report our developmental studies with these protocols as submerged entities with primary rabbit aorta smooth muscle cells for generating cell-bearing encapsulations, which demonstrate proof-of-concept for a plethora of biomedical applications. Cell viability of the post-treated cells was assessed in comparison with two controls by way of flow cytometry over a three week period, establishing a viable cellular population >70%. Hence these investigations demonstrate the ability to explore these electrified encapsulating approaches for directly forming biologically viable emulsions, which could potentially be exploited from mechanisms for cancer therapy, hormone, and diabetic treatment to applications with cosmetics. Therefore, these studies elucidate the strong implications these bio-protocols have to offer the life sciences.
电喷雾和电纺丝技术最近被率先用于直接处理活细胞。这些最新发现现在被广泛称为“生物电喷雾”和“细胞电纺丝”，已被证明在再生医学和治疗医学等生命科学领域具有巨大的应用潜力。在当前的工作中，我们报告了我们使用这些方法对原代兔主动脉平滑肌细胞进行浸没处理以生成含细胞封装体的开发研究，这为众多生物医学应用提供了概念验证。通过流式细胞术在三周时间内将处理后细胞的活力与两个对照组进行比较评估，确定存活细胞群体>70%。因此，这些研究证明了探索这些带电封装方法以直接形成具有生物活性乳液的能力，这可能从癌症治疗、激素和糖尿病治疗机制到化妆品应用等方面得到利用。因此，这些研究阐明了这些生物方法对生命科学的重大意义。
