Department of General Surgery, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
Department of Anesthesiology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
Cancer Biother Radiopharm. 2024 Apr;39(3):178-187. doi: 10.1089/cbr.2020.4042. Epub 2020 Sep 9.
A large number of studies had found that small nucleolar RNA host gene 20 (SNHG20) was a long noncoding RNA (lncRNA) that played important regulatory functions in numerous tumors. Nevertheless, the expression and pathophysiological role of SNHG20 in cholangiocarcinoma (CCA) are currently unclear. The objective of this study is to reveal the clinical significance and pathophysiological function of SNHG20 in CCA. The tumor tissues and adjacent normal tissues of CCA were obtained to determine the expression and clinical significance of SNHG20, and the targets of related genes were predicted through bioinformatics analysis. The function and regulatory mechanism of SNHG20 in CCA were evaluated by transfection, CCK-8 experiment, and luciferase reporter assay. In CCA, SNHG20 was highly expressed. Overexpressed SNHG20 was markedly interrelated with the lymph node invasion and TNM stage. In addition, it could be used as indicator to evaluate the prognosis of patients. SNHG20 sponging miR-520f-3p could accelerate the proliferation of CCA tumor cells. MiR-520f-3p acted as a tumor suppressor in CCA and could also serve as a prognostic indicator. Abolition of miR-520f-3p caused an antagonistic effect and diminished the impacts of SNHG20 knockdown. SNHG20 combined with miR-520f-3p could better predict the prognosis of CCA patients. These data confirmed the knockdown SNHG20 expression in CCA could inhibit the proliferation by means of sponging miR-520f-3p.
大量研究发现，小核仁 RNA 宿主基因 20（SNHG20）是一种长链非编码 RNA（lncRNA），在许多肿瘤中发挥重要的调控功能。然而，SNHG20 在胆管癌（CCA）中的表达和病理生理作用尚不清楚。本研究旨在揭示 SNHG20 在 CCA 中的临床意义和病理生理功能。本研究收集了 CCA 的肿瘤组织和相邻正常组织，以确定 SNHG20 的表达和临床意义，并通过生物信息学分析预测相关基因的靶基因。通过转染、CCK-8 实验和荧光素酶报告基因实验评估 SNHG20 在 CCA 中的功能和调控机制。在 CCA 中，SNHG20 呈高表达。过表达的 SNHG20 与淋巴结浸润和 TNM 分期显著相关。此外，它可以作为评估患者预后的指标。SNHG20 可通过海绵吸附 miR-520f-3p 促进 CCA 肿瘤细胞的增殖。miR-520f-3p 在 CCA 中起肿瘤抑制作用，也可以作为预后指标。抑制 miR-520f-3p 的作用可拮抗 SNHG20 敲低的影响。SNHG20 与 miR-520f-3p 联合可更好地预测 CCA 患者的预后。这些数据证实，在 CCA 中下调 SNHG20 的表达可以通过海绵吸附 miR-520f-3p 抑制增殖。
