Blair Zajdel M E, Blair G E
Department of Biochemistry, University of Leeds, UK.
Oncogene. 1988 Jun;2(6):579-84.
The intracellular distribution of the transformation-associated cellular protein p53 was studied by indirect immunofluorescence in a series of adenovirus-transformed rodent cells. In most adenovirus 2 or 5 (group C) transformed cell lines p53 was detected in discrete areas in nuclei and in all cell lines p53 was also present in a perinuclear structure. The adenovirus 2 or 5 E1B-58 kD protein, previously found to form molecular complexes with p53 in group C transformed cells, colocalized with p53 in both intracellular locations. Further studies on the region of the cell corresponding to the perinuclear body containing p53 showed that it frequently included the centrosomes of the transformed cell. The intranuclear p53 was released by mild DNAase I digestion.
通过间接免疫荧光法研究了转化相关细胞蛋白p53在一系列腺病毒转化的啮齿动物细胞中的细胞内分布。在大多数腺病毒2型或5型（C组）转化细胞系中，p53在细胞核的离散区域中被检测到，并且在所有细胞系中p53也存在于核周结构中。先前发现在C组转化细胞中与p53形成分子复合物的腺病毒2型或5型E1B-58 kD蛋白，在这两个细胞内位置都与p53共定位。对细胞中与含有p53的核周体相对应区域的进一步研究表明，它经常包括转化细胞的中心体。核内p53通过温和的DNA酶I消化而释放。
