Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, China.
Food Funct. 2020 Sep 23;11(9):8286-8296. doi: 10.1039/d0fo00999g.
Banana is an essential food resource in many tropical and subtropical countries. Metabolites in banana greatly influence its nutritional value and flavor. However, metabolic changes that occur in different developmental stages have not been comprehensively evaluated. In this study, widely targeted metabolomics based on multiple reaction monitoring was used in investigating dynamic changes in metabolites at three stages of fruit development. A total of 655 metabolites were identified in all the stages. A hierarchical cluster analysis of metabolites showed six clear expression patterns at the three developmental stages, and 69 up-regulated differential metabolites were identified in mature fruits compared with young and mature green fruits. A metabolic pathway analysis of differential metabolites showed significant enrichment of the flavonoid biosynthesis pathway and the phenylalanine, tyrosine, and tryptophan biosynthesis pathways. These results may serve as a reference for the isolation and identification of functional compounds from banana and for their sufficient utilization in the future.
香蕉是许多热带和亚热带国家的重要食物资源。香蕉中的代谢物极大地影响其营养价值和风味。然而,不同发育阶段发生的代谢变化尚未得到全面评估。在这项研究中,基于多重反应监测的广泛靶向代谢组学被用于研究果实发育三个阶段代谢物的动态变化。在所有阶段共鉴定出 655 种代谢物。对代谢物的层次聚类分析显示,在三个发育阶段有六种明确的表达模式,与青果和成熟绿果相比,成熟果实中有 69 种上调差异代谢物。差异代谢物的代谢途径分析显示,类黄酮生物合成途径和苯丙氨酸、酪氨酸和色氨酸生物合成途径显著富集。这些结果可为香蕉功能化合物的分离鉴定及其未来的充分利用提供参考。