Department of Pharmacology, Medical Research Division, 68787National Research Centre, Dokki, Giza, Egypt.
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Hum Exp Toxicol. 2021 Mar;40(3):526-537. doi: 10.1177/0960327120954522. Epub 2020 Sep 10.
Acute paracetamol over dose-induced hepatotoxicity is considered an important medical hazard especially among women. Omega-3 long-chain polyunsaturated fatty acids (Omega-3 PUFAs) daily doses are nowadays recommended for their antioxidant and anti-inflammatory potentials. Fourier transform infrared (FTIR) spectroscopy is considered a reliable method in analyzing cellular alterations and is now efficiently used to diagnose several diseases and the efficacy of drugs even in the early stages. The aim of our study was to evaluate the hepatoprotective effect of Omega-3 PUFAs against paracetamol-induced hepatotoxicity in rats confirmed through measuring protein alterations in hepatocytes by FTIR. Rats were pretreated with Omega-3 PUFAs (50 and 100 mg/kg) for 21 days prior to oral ingestion of paracetamol. FTIR results revealed that Omega-3 PUFAs (50 mg/kg) limited the toxic effects of paracetamol by restoring the hepatic amide I to amide II ratio. In addition; biochemical analyses demonstrated that serum ALT, AST, Cholesterol, LDL-cholesterol and Il-6 levels as well as hepatic TNF-α, MDA, NOx levels were decreased. Besides; serum HDL-cholesterol level and hepatic GSH level were increased. Histopathological examinations of hepatic sections validated the hepatoprotective potential. The overall effect of this dose was comparable to those of the usual recommended hepatoprotective supplement; silymarin. In conclusion; it would be recommended to use Omega-3 PUFAs in low doses on daily bases as a hepatoprotective agent.
急性对乙酰氨基酚过量诱导的肝毒性被认为是一种重要的医学危害，尤其是在女性中。如今，建议每日摄入欧米伽-3 长链多不饱和脂肪酸（欧米伽-3 PUFA），因为其具有抗氧化和抗炎潜力。傅里叶变换红外（FTIR）光谱被认为是分析细胞变化的可靠方法，现在可用于诊断多种疾病和药物的疗效，甚至在早期阶段。我们的研究旨在通过 FTIR 测量肝细胞中蛋白质的变化来评估欧米伽-3 PUFA 对乙酰氨基酚诱导的肝毒性的保护作用。大鼠在口服对乙酰氨基酚前 21 天预先用欧米伽-3 PUFA（50 和 100mg/kg）预处理。FTIR 结果表明，欧米伽-3 PUFA（50mg/kg）通过恢复肝酰胺 I 与酰胺 II 的比值，限制了对乙酰氨基酚的毒性作用。此外；生化分析表明，血清 ALT、AST、胆固醇、LDL-胆固醇和 Il-6 水平以及肝 TNF-α、MDA、NOx 水平降低。此外；血清 HDL-胆固醇水平和肝 GSH 水平升高。肝切片的组织病理学检查验证了其潜在的保肝作用。这种剂量的整体效果与通常推荐的保肝补充剂水飞蓟素相当。总之；建议每日低剂量使用欧米伽-3 PUFA 作为保肝剂。
