Genomics Group, Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
Department of Cellular and Molecular Medicine, University of Copenhagen, 2400 Copenhagen, Denmark.
RNA. 2020 Dec;26(12):1919-1934. doi: 10.1261/rna.076760.120. Epub 2020 Sep 10.
During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2'--Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalog of 2'--Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.
在斑马鱼发育过程中，一种早期类型的 rRNA 逐渐被序列差异较大的晚期类型取代。我们应用 RiboMeth-seq 对发育阶段的 rRNA 进行 2'--Me 谱分析，以了解甲基化模式的变化是否是这种转变的一个组成部分。我们编制了一个包含 98 个高可信度位点的 2'--Me 位点和框 C/D 指导 RNA 目录，其中包括 10 个在其他脊椎动物中未知的位点，其中一个位点是晚期 rRNA 特有的。我们确定了在发育过程中甲基化状态发生变化的亚组位点，并发现其中一些可以用其同源 SNORD 的可用性来解释。在发育过程中发生变化的位点在斑马鱼中揭示的新位点中富集。我们提出，早期类型的 rRNA 是一种特殊形式，其结构和核糖甲基化模式可能是对发育特征的一种适应，包括特定母体 mRNA 的翻译。
