Purification and characterisation of prostaglandin endoperoxide D-isomerase, a cytoplasmic, glutathione-requiring enzyme.

Prostaglandin endoperoxide D-isomerase in rat spleen was purified until homogeneity. This cytoplasmic enzyme occurs in many organs of the rat and also in other species, and requires specifically glutathione for its action. The molecular weight is 30 000 and the isoelectric point pI 5.2.