Zhou Danni, Sun Yaxun, Shen Jiaxi, Su Jun, Gong Tingyu, Jiang Chenyang, Liang Ping
Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China.
Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
Stem Cell Res. 2020 Oct;48:101965. doi: 10.1016/j.scr.2020.101965. Epub 2020 Aug 27.
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited disease in which the right ventricular myocardium is replaced by progressive fibrous adipose tissue. ARVC is clinically characterized by right ventricular enlargement, ventricular arrhythmia, and sudden cardiac death. It eventually leads to heart failure, and thus has a significant impact on the patient's health. In this study, human dermal fibroblasts were obtained from a patient with ARVC, which were subsequently reprogrammed with a non-integrated Sendai virus to generate a patient-specific induced pluripotent stem cell (iPSC) line. The iPSC line exhibited normal karyotype and morphology, expressed pluripotency markers, and was capable of differentiating into three germ layers.
致心律失常性右室心肌病（ARVC）是一种遗传性疾病，其中右心室心肌被进行性纤维脂肪组织替代。ARVC的临床特征为右心室扩大、室性心律失常和心源性猝死。它最终会导致心力衰竭，因此对患者健康有重大影响。在本研究中，从一名ARVC患者获取人皮肤成纤维细胞，随后用无整合的仙台病毒对其进行重编程，以生成患者特异性诱导多能干细胞（iPSC）系。该iPSC系表现出正常的核型和形态，表达多能性标志物，并且能够分化为三个胚层。
