School of Resources and Environmental Engineering, Anhui University, Hefei, 230601, China.
Key Laboratory of Wetland Ecological Protection and Restoration, Hefei, China.
Environ Sci Pollut Res Int. 2021 Jan;28(3):3375-3385. doi: 10.1007/s11356-020-10700-8. Epub 2020 Sep 11.
Phragmites australis, which is widely distributed throughout the world, is often used in the phytoremediation of acid mine drainage (AMD) due to its various mechanisms for survival under extremely harsh conditions. To explore the different responses of different aerial organs of P. australis to stress, soil and plant samples were collected from the AMD-polluted area of the Tongling mining area. The contents of manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), and lead (Pb) in the soil and the leaf blades, leaf sheaths, and stems of P. australis as well as the contents/activities of cysteine synthase (CSase), superoxide dismutase (SOD), peroxidase (POD), glutathione (GSH), malondialdehyde (MDA), and proline (Pro) in the organs were determined. Our results revealed that the leaf sheath had the highest potential to store metals of all the organs. The highest translocation factor (TF) for Fe was observed from the stems to the leaf sheaths. A higher bioconcentration factor (BCF) for Mn was found in the leaf blades and leaf sheaths, while higher BCFs for Cd and Zn were observed in the stems. The content/activity of enzymatic and non-enzymatic stress-resistance substances varied from organ to organ. In general, the leaf sheaths remained almost as or slightly less stress-resistant than the leaf blades. It can be concluded that different plant organs play different roles in stress resistance, and understanding the tolerance mechanism of leaf sheaths to metals is essential for the application of phytoremediation procedures.
芦苇（Phragmites australis）分布广泛，由于其在极端恶劣条件下生存的多种机制，常用于酸性矿山排水（AMD）的植物修复。为了探究不同地上器官对胁迫的不同响应，从铜陵矿区 AMD 污染区采集土壤和植物样品。测定土壤和芦苇叶片、叶鞘和茎中的锰（Mn）、铁（Fe）、铜（Cu）、锌（Zn）、砷（As）、镉（Cd）和铅（Pb）含量，以及器官中半胱氨酸合酶（CSase）、超氧化物歧化酶（SOD）、过氧化物酶（POD）、谷胱甘肽（GSH）、丙二醛（MDA）和脯氨酸（Pro）的含量/活性。结果表明，所有器官中叶鞘对金属的储存潜力最大。Fe 的最高迁移系数（TF）从茎转移到叶鞘。Mn 的生物浓缩系数（BCF）在叶片和叶鞘中较高，而 Cd 和 Zn 的 BCF 则在茎中较高。酶和非酶应激抗性物质的含量/活性因器官而异。一般来说，叶鞘的应激抗性几乎与叶片相同或略低。可以得出结论，不同的植物器官在应激抵抗中发挥不同的作用，了解叶鞘对金属的耐受机制对于植物修复程序的应用至关重要。
