Žlajpah Margareta, Boštjančič Emanuela, Zidar Nina
Institute of Pathology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.
Epigenomics. 2020 Aug;12(16):1389-1403. doi: 10.2217/epi-2020-0032. Epub 2020 Sep 14.
To identify (epi)genetic regulators of osteopontin (OPN, encoded by gene) from normal colon mucosa to adenoma, adenoma with early carcinoma and advanced carcinoma. Biopsy samples of 41 patients with different patohistologic diagnosis were used. Using qPCR, pyrosequencing and statistical analysis, we determined the expression level of osteopontin regulatory miRNAs, its copy number and methylation status. We showed that expression is inversely proportional to the expression level of and that expression might be also controlled by copy number and methylation. These results suggest that not only expression of but also its copy number, methylation status and expression of its regulators might be used as a potential biomarker of colorectal cancer.
为了鉴定从正常结肠黏膜到腺瘤、早期癌腺瘤和晚期癌中骨桥蛋白（由基因编码的OPN）的（表观）遗传调节因子。使用了41例不同病理组织学诊断患者的活检样本。通过定量聚合酶链反应（qPCR）、焦磷酸测序和统计分析，我们确定了骨桥蛋白调节性微小核糖核酸（miRNAs）的表达水平、其拷贝数和甲基化状态。我们发现的表达与的表达水平呈负相关，并且的表达可能也受拷贝数和甲基化的控制。这些结果表明，不仅的表达，而且其拷贝数、甲基化状态及其调节因子的表达都可能用作结直肠癌的潜在生物标志物。
