Gross Jürgen H
Institute of Organic Chemistry, Heidelberg University, Heidelberg, Germany.
Eur J Mass Spectrom (Chichester). 2020 Oct;26(5):324-331. doi: 10.1177/1469066720958535. Epub 2020 Sep 13.
In positive-ion direct analysis in real time-mass spectrometry (DART-MS), mono-, di, and trisaccharides form [M+NH] ions. Some of them, in addition, yield abundant [M+NH] cluster ions (n = 1-6)), and thus, can serve for mass calibration. Saccharose, CHO, the most common sugar, also termed sucrose, is among the [M+NH] cluster ion forming species. Saccharose may therefore be employed as a cheap and ubiquitous mass calibration standard. The extent of saccharose cluster ion formation depends on the temperature of the DART gas, sample load, and instrumental parameters like trapping conditions of ions prior to mass analysis. This study identifies optimized experimental conditions and demonstrates the application of saccharose cluster ion-based mass calibration for accurate mass measurements in DART mode on a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer.
在正离子实时直接分析质谱法（DART-MS）中，单糖、二糖和三糖会形成[M+NH]离子。此外，其中一些还会产生大量的[M+NH]簇离子（n = 1 - 6），因此可用于质量校准。蔗糖，化学式为C₁₂H₂₂O₁₁，是最常见的糖类，也称为白砂糖，属于能形成[M+NH]簇离子的物质。因此，蔗糖可用作一种廉价且普遍存在的质量校准标准物。蔗糖簇离子的形成程度取决于DART气体的温度、样品负载量以及诸如质量分析前离子的捕获条件等仪器参数。本研究确定了优化的实验条件，并展示了基于蔗糖簇离子的质量校准在傅里叶变换离子回旋共振（FT-ICR）质谱仪上以DART模式进行精确质量测量中的应用。
