Luo Ming, Tang Xiangyue, Zhu Juehua, Qiu Zhihua, Jiang Yongjun
The Second Affiliated Hospital of Guangzhou Medical University.
Department of Neurology, The First Affiliated Hospital of Soochow University.
J Vis Exp. 2020 Aug 27(162). doi: 10.3791/60783.
Pontine infarction is the most common stroke subtype in the posterior circulation, while there lacks a rodent model mimicking pontine infarction. Provided here is a protocol for successfully establishing a rat model of acute pontine infarction. Rats weighing about 250 g are used, and a probe with an insulated sheath is injected into the pons using a stereotaxic apparatus. A lesion is produced by the electrical stimulation with a single pulse. The Longa score, Berderson score, and beam balance test are used to assess neurological deficits. Additionally, the adhesive-removal somatosensory test is used to determine sensorimotor function, and the limb placement test is used to evaluate proprioception. MRI scans are then used to assess the infarction in vivo, and TTC staining is used to confirm the infarction in vitro. Here, a successful infarction is identified that is located in the anterolateral basis of the rostral pons. In conclusion, a new method is described to establish an acute pontine infarction rat model.
脑桥梗死是后循环中最常见的卒中亚型，但缺乏模拟脑桥梗死的啮齿动物模型。本文提供了一种成功建立大鼠急性脑桥梗死模型的方案。使用体重约250g的大鼠，通过立体定位仪将带有绝缘鞘的探针注入脑桥。通过单次电刺激产生损伤。使用Longa评分、Berderson评分和光束平衡试验评估神经功能缺损。此外，采用粘胶去除体感试验确定感觉运动功能，采用肢体放置试验评估本体感觉。然后使用MRI扫描在体内评估梗死情况，使用TTC染色在体外确认梗死情况。在此，确定了一个成功的梗死灶位于脑桥嘴侧前外侧基底。总之，描述了一种建立急性脑桥梗死大鼠模型的新方法。
