一项比较下一代自动化细胞计数器检测外周血中造血祖细胞计数与流式细胞术 CD34 计数，以及从自体和异体供者采集的外周血造血干细胞移植的研究。

A study to compare Hematopoietic Progenitor Cell count determined on a next-generation automated cell counter with flow cytometric CD34 count in peripheral blood and the harvested peripheral blood stem cell graft from autologous and allogenic donors.

机构信息

Department of Transfusion Medicine and Immuno-haematology, Vellore, India.

Department of Haematology, Christian Medical College and Hospital, Vellore, India.

出版信息

Int J Lab Hematol. 2021 Feb;43(1):76-83. doi: 10.1111/ijlh.13341. Epub 2020 Sep 14.

DOI:10.1111/ijlh.13341

PMID:32926561

Abstract

INTRODUCTION

A successful bone marrow transplant requires a minimum of 2-4 × 10 cells/kg patient body weight of CD 34+ cells to be transfused, where peripheral blood CD34+ cell count being and ideal predictor. We compared the correlation and predictive capacity of both hematopoietic progenitor cell count (HPC) determined on the Sysmex XN-9000 and flow cytometric CD34 in autologous and allogenic donors.

METHODS

Autologous and allogenic donors were taken as per criteria. TLC (Total Leukocyte Count), MNC (Mononuclear cell count), HPC, and CD34 assay were done in both the peripheral blood prior to apheresis, and the harvest product postapheresis. Sysmex XN-9000 was used for TLC, MNC, and HPC tests, and a modified ISH-AGE protocol was used to enumerate CD34 by flow cytometry. Statistical analysis was done using SPSS 16.0.

RESULTS

Sixty-seven allogenic and 35 autologous donors were enrolled. 45% were females, and 55% were males. Correlation between HPC and CD34 was found to be 0.887 with P value < .01 in peripheral blood and 0.847 with P value < .01 in the harvested product. On the other hand, TLC had a correlation of 0.424 and 0.520 in peripheral blood and harvested, respectively. MNC had a weak association. The cutoff value for a target dose of 2 × 10 CD34 cells/kg was 37 × 10 /L for pre-HPC. For a target of 4 × 10 CD34 cells/kg, the cutoff value calculated to be 54 × 10 /L (Sensitivity: 85%, Specificity: 89%) for peripheral blood HPC.

CONCLUSION

We conclude that HPC is comparable to CD34 in predicting harvest product's adequacy.

摘要

简介

成功的骨髓移植需要输注至少 2-4×10 个细胞/kg 患者体重的 CD34+细胞，外周血 CD34+细胞计数是理想的预测指标。我们比较了自体和同种异体供体的 Sysmex XN-9000 上测定的造血祖细胞计数（HPC）与流式细胞术 CD34 的相关性和预测能力。

方法

按照标准采集自体和同种异体供者。在采集前和采集后，分别对外周血进行总白细胞计数（TLC）、单核细胞计数（MNC）、HPC 和 CD34 检测。Sysmex XN-9000 用于 TLC、MNC 和 HPC 检测，流式细胞术计数 CD34 使用改良的 ISH-AGE 方案。使用 SPSS 16.0 进行统计分析。

结果

共纳入 67 例同种异体和 35 例自体供者。45%为女性，55%为男性。外周血中 HPC 与 CD34 之间的相关性为 0.887，P 值<.01；采集物中 HPC 与 CD34 之间的相关性为 0.847，P 值<.01。另一方面，TLC 在外周血和采集物中的相关性分别为 0.424 和 0.520。MNC 相关性较弱。目标剂量为 2×10 CD34 个细胞/kg 时，HPC 的预检测截止值为 37×10 /L。目标剂量为 4×10 CD34 个细胞/kg 时，计算出外周血 HPC 的截止值为 54×10 /L（灵敏度：85%，特异性：89%）。