Yamane T, Hopfield J J
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2246-50. doi: 10.1073/pnas.74.6.2246.
The enzymatic aminoacylation of tRNA can be viewed as a means of proofreading either the amino acid or the tRNA or both. We have conducted further experimental tests of kinetic proofreading in discriminating between cognate and noncognate amino acids and tRNAs as follows: (formula: see text). In cases (i) and (ii) the amino acids are proofread, in cases (iii) and (iv) the tRNA is proofread, and in case (v), both the amino acid and the tRNA are proofread. ATP consumed per acylation was 400, 1.5, 40, 25, and 1000, respectively. High ATP/aminoacylation ratios are diagnostic for kinetic proofreading.
tRNA的酶促氨酰化可被视为对氨基酸或tRNA或两者进行校对的一种方式。我们进行了进一步的动力学校对实验测试，以区分同源和非同源氨基酸及tRNA，具体如下：（公式：见正文）。在情况（i）和（ii）中对氨基酸进行校对，在情况（iii）和（iv）中对tRNA进行校对，而在情况（v）中，氨基酸和tRNA都进行校对。每次酰化消耗的ATP分别为400、1.5、40、25和1000。高ATP/酰化比率是动力学校对的诊断依据。
