合成酶对tRNA进行氨酰化过程中动力学校正的实验证据。
Experimental evidence for kinetic proofreading in the aminoacylation of tRNA by synthetase.
作者信息
Yamane T, Hopfield J J
出版信息
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2246-50. doi: 10.1073/pnas.74.6.2246.
Abstract
The enzymatic aminoacylation of tRNA can be viewed as a means of proofreading either the amino acid or the tRNA or both. We have conducted further experimental tests of kinetic proofreading in discriminating between cognate and noncognate amino acids and tRNAs as follows: (formula: see text). In cases (i) and (ii) the amino acids are proofread, in cases (iii) and (iv) the tRNA is proofread, and in case (v), both the amino acid and the tRNA are proofread. ATP consumed per acylation was 400, 1.5, 40, 25, and 1000, respectively. High ATP/aminoacylation ratios are diagnostic for kinetic proofreading.
摘要
tRNA的酶促氨酰化可被视为对氨基酸或tRNA或两者进行校对的一种方式。我们进行了进一步的动力学校对实验测试，以区分同源和非同源氨基酸及tRNA，具体如下：（公式：见正文）。在情况（i）和（ii）中对氨基酸进行校对，在情况（iii）和（iv）中对tRNA进行校对，而在情况（v）中，氨基酸和tRNA都进行校对。每次酰化消耗的ATP分别为400、1.5、40、25和1000。高ATP/酰化比率是动力学校对的诊断依据。
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本文引用的文献
1
Purification and substrate specificity of a phenylalanine-activating enzyme from Escherichia coli 9723.来自大肠杆菌9723的苯丙氨酸激活酶的纯化及底物特异性
J Biol Chem. 1962 Sep;237:2850-4.
2
The specificity of protein biosynthesis.蛋白质生物合成的特异性
Adv Protein Chem. 1959;14:115-73. doi: 10.1016/s0065-3233(08)60610-5.
3
Recognition of tRNA by isoleucyl-tRNA synthetase. Effect of substrates on the dynamics of tRNA-enzyme interaction.异亮氨酰 - tRNA合成酶对tRNA的识别。底物对tRNA - 酶相互作用动力学的影响。
J Mol Biol. 1969 Jun 14;42(2):171-89. doi: 10.1016/0022-2836(69)90037-0.
4
Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.在聚氯三氟乙烯支持的反相色谱柱上改进转运RNA的分离。
Biochim Biophys Acta. 1971 Feb 11;228(3):770-4. doi: 10.1016/0005-2787(71)90748-9.
5
Transfer ribonucleic acid-induced hydrolysis of valyladenylate bound to isoleucyl ribonucleic acid synthetase.转移核糖核酸诱导与异亮氨酰核糖核酸合成酶结合的缬氨酰腺苷酸水解。
J Biol Chem. 1966 Feb 25;241(4):839-45.
6
Purification and properties of isoleucyl ribonucleic acid synthetase from Escherichia coli.大肠杆菌异亮氨酰核糖核酸合成酶的纯化及性质
J Biol Chem. 1966 Feb 25;241(4):831-8.
7
Purification and physical characterization of tyrosyl ribonucleic acid synthetases from Escherichia coli and Bacillus subtilis.来自大肠杆菌和枯草芽孢杆菌的酪氨酰核糖核酸合成酶的纯化及物理特性研究
Biochemistry. 1966 May;5(5):1681-90. doi: 10.1021/bi00869a033.
8
Fluorescence studies of interactions between Escherichia coli valyl-tRNA synthetase and its substrates.
J Mol Biol. 1971 May 28;58(1):349-56. doi: 10.1016/0022-2836(71)90251-8.
9
Studies on the purification and properties of factor Tu from E. coli.大肠杆菌中Tu因子的纯化及性质研究
Arch Biochem Biophys. 1970 Nov;141(1):26-37. doi: 10.1016/0003-9861(70)90102-5.
10
Studies on valyl-tRNA synthetase and tRNA from Escherichia coli. I. Purification and properties of the enzyme from normal Escherichia coli strains.大肠杆菌缬氨酰 - tRNA合成酶与tRNA的研究。I. 正常大肠杆菌菌株中该酶的纯化及性质
J Mol Biol. 1969 Aug 28;44(1):1-15. doi: 10.1016/0022-2836(69)90401-x.
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