Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
Int J Mol Sci. 2020 Sep 10;21(18):6608. doi: 10.3390/ijms21186608.
Sucrose, an important sugar, is transported from source to sink tissues through the phloem, and plays important role in the development of important traits in plants. However, the gene family is still not well characterized in pomegranate. In this study, we first identified the pomegranate sucrose transporter ( gene family from the whole genome. Then, the phylogenetic relationship of genes, gene structure and their promoters were analyzed. Additionally, their expression patterns were detected during the development of the seed. Lastly, genetic transformation and cytological observation were used to study the function of . A total of ten pomegranate genes were identified from the whole genome of pomegranate 'Tunisia'. The promoter region of all the pomegranate genes contained myeloblastosis (MYB) elements. Four of the genes, , , and , were differentially expressed during seed development. We further noticed that was expressed most prominently in the stem parts in transgenic plants compared to other tissue parts (leaves, flowers and silique). The cells in the xylem vessels were small and lignin content was lower in the transgenic plants as compared to wild plants. In general, our result suggests that the MYB cis-elements in the promoter region might regulate expression to control the structure of xylem, thereby affecting seed hardness in pomegranate.
蔗糖是一种重要的糖，通过韧皮部从源组织运输到汇组织，并在植物重要性状的发育中发挥重要作用。然而，在石榴中，基因家族仍未得到很好的描述。在这项研究中，我们首先从全基因组中鉴定了石榴蔗糖转运体（基因家族。然后，分析了基因的系统发育关系、基因结构及其启动子。此外，还检测了它们在种子发育过程中的表达模式。最后，通过遗传转化和细胞学观察来研究的功能。我们从石榴‘突尼斯’的全基因组中鉴定出了 10 个石榴基因。所有石榴基因的启动子区域都含有髓样细胞瘤（MYB）元件。在种子发育过程中，有 4 个基因（、、和）表现出差异表达。我们进一步注意到，与其他组织部位（叶片、花朵和角果）相比，在转基因植物的茎部中表达最为显著。与野生型植物相比，转基因植物木质部导管细胞较小，木质素含量较低。总的来说，我们的结果表明，启动子区域中的 MYB 顺式元件可能调节基因的表达，从而控制木质部的结构，进而影响石榴种子的硬度。
