State Key Laboratory of Phytochemistry and Plant Resources in West China, and CAS Center for Excellence in Molecular Plant Sciences, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China.
College of Chemistry and Pharmacy, Northwest Agriculture and Forestry University, Yangling 712100, China.
Chin J Nat Med. 2020 Sep;18(9):677-683. doi: 10.1016/S1875-5364(20)60006-1.
Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B (1) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the N-labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed via an oxidative release of the assembly chain assisted by the P450 domain.
因替米星是一种含有末端羧酰胺基和三烯链的聚酮类抗生素。从链霉菌 pactum L8 的粗提物中分离得到因替米星 B（1）及其两个新类似物 2 和 3。描述了因替米星生物合成的基因簇的鉴定以及 N 标记甘氨酸掺入因替米星的情况。结合 NRPS 模块中罕见 P450 结构域的基因缺失，通过 P450 结构域辅助的组装链氧化释放，提出了因替米星中羧酰胺部分的形成机制。
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