Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Arab J Gastroenterol. 2021 Mar;22(1):34-39. doi: 10.1016/j.ajg.2020.08.006. Epub 2020 Sep 11.
Obstructive cholestasis increases the levels of oxidants and inflammatory mediators, leading to liver damage. Previous studies have found that Cichorium intybus possesses anti-inflammatory effects. In the present study, the effects of the hydroalcoholic extract of C. intybus leaves were assessed in a rat model of obstructive cholestasis.
Male Wistar rats were randomly divided into five groups (n = 6 rats per group): sham-operated, control [bile duct ligation (BDL) + vehicle)] and BDL + extract treatment (100, 200 and 400 mg/kg/day, i.p.) groups. Rats received treatments for 7 consecutive days. On the eighth day, prothrombin time (PT); serum albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase and total and direct bilirubin levels and total antioxidant and paraoxonase activities were measured using colorimetric methods. In addition, tumour necrosis factor-α and nitric oxide (NO) levels were measured using enzyme-linked immunosorbent assay.
The hydroalcoholic extract of C. intybus significantly decreased PT and the serum levels of AST, ALT, TNF-α and NO compared with the control group (p < 0.05). On the other hand, the serum albumin levels were increased in the extract-treated groups compared with the control group (p < 0.05).
The hydroalcoholic extract of C. intybus protects the liver against injury induced by obstructive cholestasis.
阻塞性胆汁淤积会增加氧化剂和炎症介质的水平，导致肝脏损伤。先前的研究发现，菊苣具有抗炎作用。在本研究中，评估了菊苣叶水醇提取物在阻塞性胆汁淤积大鼠模型中的作用。
雄性 Wistar 大鼠随机分为五组（每组 6 只大鼠）：假手术组、对照组[胆管结扎（BDL）+载体]和 BDL+提取物治疗组（100、200 和 400mg/kg/天，腹腔注射）。大鼠连续治疗 7 天。第 8 天，使用比色法测定凝血酶原时间（PT）；血清白蛋白、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶和总胆红素及直接胆红素水平以及总抗氧化和对氧磷酶活性。此外，使用酶联免疫吸附测定法测定肿瘤坏死因子-α和一氧化氮（NO）水平。
菊苣水醇提取物与对照组相比，显著降低了 PT 以及血清 AST、ALT、TNF-α 和 NO 水平（p<0.05）。另一方面，与对照组相比，提取物治疗组的血清白蛋白水平升高（p<0.05）。
菊苣水醇提取物可保护肝脏免受阻塞性胆汁淤积引起的损伤。
