Dulundu Ender, Ozel Yahya, Topaloglu Umit, Toklu Hale, Ercan Feriha, Gedik Nursal, Sener Goksel
Department of Fifth Surgery, Haydarpasa Numune Education and Research Hospital, Istanbul, Turkey.
J Gastroenterol Hepatol. 2007 Jun;22(6):885-92. doi: 10.1111/j.1440-1746.2007.04875.x.
The aim of this study was to assess the protective effect of grape seed extract (GSE) against oxidative liver injury and fibrosis induced by biliary obstruction in rats.
Wistar albino rats were divided into four groups; control (C), GSE-treated, bile duct ligated (BDL), and BDL and GSE-treated (BDL + GSE) groups. GSE was administered at a dose of 50 mg/kg a day orally for 28 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels were determined to assess liver function and tissue damage, respectively. Tumor necrosis factor-alpha (TNF-alpha) and antioxidant capacity (AOC) were assayed in plasma samples. Liver tissues were taken for determination of the hepatic malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen content. Production of reactive oxidants was monitored by chemiluminescence (CL) assay.
Serum AST, ALT, LDH and plasma TNF-alpha were elevated in the BDL group as compared to the control group and were significantly decreased with GSE treatment. Plasma AOC and hepatic GSH level, depressed by BDL, was elevated back to the control level in the GSE-treated BDL group. Increases in tissue MDA level, MPO activity and collagen content due to BDL were also attenuated by GSE treatment. Furthermore, luminol and lucigenin CL values in the BDL group increased dramatically compared to the control and were reduced by GSE treatment.
These results suggest that GSE protects the liver from oxidative damage following bile duct ligation in rats. This effect possibly involves the inhibition of neutrophil infiltration and lipid peroxidation; thus, restoration of oxidant and antioxidant status in the tissue.
本研究旨在评估葡萄籽提取物（GSE）对大鼠胆管梗阻所致氧化肝损伤和肝纤维化的保护作用。
将Wistar白化大鼠分为四组：对照组（C）、GSE处理组、胆管结扎组（BDL）以及BDL + GSE处理组。GSE以50 mg/kg的剂量每日口服给药，持续28天。分别测定血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和乳酸脱氢酶（LDH）水平以评估肝功能和组织损伤。检测血浆样本中的肿瘤坏死因子-α（TNF-α）和抗氧化能力（AOC）。取肝组织测定肝丙二醛（MDA）和谷胱甘肽（GSH）水平、髓过氧化物酶（MPO）活性及胶原蛋白含量。通过化学发光（CL）测定法监测活性氧化剂的产生。
与对照组相比，BDL组血清AST、ALT、LDH及血浆TNF-α升高，而GSE处理后显著降低。BDL降低的血浆AOC和肝GSH水平在GSE处理的BDL组中回升至对照水平。BDL所致组织MDA水平、MPO活性及胶原蛋白含量的增加也因GSE处理而减弱。此外，与对照组相比，BDL组的鲁米诺和光泽精CL值显著升高，而GSE处理使其降低。
这些结果表明，GSE可保护大鼠胆管结扎后的肝脏免受氧化损伤。这种作用可能涉及抑制中性粒细胞浸润和脂质过氧化；从而恢复组织中的氧化剂和抗氧化剂状态。
