Risau W, Sweet E, D'Amore P A
Max-Planck-Institut fur Entwicklungsbiologie, Tubingen.
Microvasc Res. 1988 May;35(3):265-77. doi: 10.1016/0026-2862(88)90081-7.
Monoclonal antibodies have been raised against cell surface proteins of cultured bovine retinal pericytes. One antibody was selected, designated PC4, which preferentially stained primary cultures of bovine pericytes and smooth muscle cells, but not endothelial cells and fibroblasts. In freshly plated cells a homogeneous cell surface staining was observed, whereas in well-spread cells the antigen was concentrated at cell attachment sites. The antigen remained at these sites after spontaneous detachment of the cells. PC4 monoclonal antibodies reacted with a major protein of 130,000 Da and two minor antigens of 75,000 and 70,000 Da in immunoblots of extracts from cultured pericytes and smooth muscle cells and from fibroblasts cultured for an extended period of time. In frozen sections of bovine tissues the antigen was found in the vascular wall. There was no staining of skeletal muscle cells or duodenal smooth muscle cells, indicating that the antigen may be a specific component of the vascular wall.
已制备出针对培养的牛视网膜周细胞细胞表面蛋白的单克隆抗体。选择了一种名为PC4的抗体，它优先标记牛周细胞和平滑肌细胞的原代培养物，但不标记内皮细胞和成纤维细胞。在刚接种的细胞中观察到均匀的细胞表面染色，而在铺展良好的细胞中，抗原集中在细胞附着部位。细胞自发脱离后，抗原仍留在这些部位。在培养的周细胞、平滑肌细胞以及长时间培养的成纤维细胞提取物的免疫印迹中，PC4单克隆抗体与一种130,000道尔顿的主要蛋白以及两种75,000和70,000道尔顿的次要抗原发生反应。在牛组织的冰冻切片中，在血管壁中发现了该抗原。骨骼肌细胞或十二指肠平滑肌细胞没有染色，这表明该抗原可能是血管壁的一种特异性成分。
