铜绿假单胞菌感染通过调节性 T 细胞的激活来损害 NKG2D 依赖性 NK 细胞的细胞毒性。
Pseudomonas aeruginosa Infection Impairs NKG2D-Dependent NK Cell Cytotoxicity through Regulatory T-Cell Activation.
机构信息
Université de Nantes, Thérapeutiques Cliniques et Expérimentales des Infections, EA 3826, Institut de Recherche en Santé 2 Nantes Biotech, Nantes, France.
Intensive Care Unit, Anesthesia and Critical Care Department, Hôtel Dieu, University Hospital of Nantes, Nantes, France.
出版信息
Infect Immun. 2020 Nov 16;88(12). doi: 10.1128/IAI.00363-20.
Natural killer (NK) cells play a key role in both antibacterial and antitumor immunity. infection has already been reported to alter NK cell functions. We studied the effect of on NK cell cytotoxic response (CD107a membrane expression) to a lymphoma cell line. Through positive and negative cell sorting and adoptive transfer, we determined the influence of monocytes, lymphocytes, and regulatory T cells (Treg) on NK cell function during infection. We also studied the role of the activating receptor natural killer group 2D (NKG2D) in NK cell response to B221. We determined that significantly altered both cytotoxic response to B221 and NKG2D expression on NK cells in a Treg-dependent manner and that the NKG2D receptor was involved in NK cell cytotoxic response to B221. Our results also suggested that during infection, monocytes participated in Treg-mediated NK cell alteration. In conclusion, infection impairs NK cell cytotoxicity and alters antitumor immunity. These results highlight the strong interaction between bacterial infection and immunity against cancer.
自然杀伤 (NK) 细胞在抗菌和抗肿瘤免疫中发挥着关键作用。感染已被报道会改变 NK 细胞的功能。我们研究了对淋巴瘤细胞系的 NK 细胞细胞毒性反应（CD107a 膜表达）的影响。通过阳性和阴性细胞分选和过继转移，我们确定了单核细胞、淋巴细胞和调节性 T 细胞 (Treg) 在感染过程中对 NK 细胞功能的影响。我们还研究了激活受体自然杀伤组 2D (NKG2D) 在 NK 细胞对 B221 反应中的作用。我们确定，以 Treg 依赖性方式显著改变了 NK 细胞对 B221 的细胞毒性反应和 NKG2D 表达，并且 NKG2D 受体参与了 NK 细胞对 B221 的细胞毒性反应。我们的结果还表明，在感染期间，单核细胞参与了 Treg 介导的 NK 细胞改变。总之，感染会损害 NK 细胞的细胞毒性，并改变抗肿瘤免疫。这些结果强调了细菌感染与癌症免疫之间的强烈相互作用。
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