ESKAPE 组细菌对自然杀伤细胞特性的调节

Modulation of NK Cell Properties by ESKAPE Group Bacteria.

作者信息

Grebenkina Polina, Juchina Varvara, Tyshchuk Elizaveta, Gulina Ananstasia, Denisova Elizaveta, Korobova Zoia, Orlov Sergey, Totolian Areg, Kraeva Lyudmila, Sokolov Dmitry

机构信息

Saint-Petersburg Pasteur Institute, St. Petersburg 197101, Russia.

Research Institute of Obstetrics and Gynecology named after D.O. Ott, St. Petersburg 199034, Russia.

出版信息

Int J Mol Sci. 2025 Aug 30;26(17):8449. doi: 10.3390/ijms26178449.

Functions of natural killer cells (NK cells) can be modulated by environmental stimuli. However, the role of bacterial components in this modulation remains an area of ongoing research. This study investigates how bacterial supernatants influence NK cell function-including cytotoxicity, phenotype, and cytokine and mRNA production-following co-culture. These parameters were measured by flow cytometry and RT-PCR. We found that NK cells express TLR2 and TLR5, and that exposure to supernatants from ESKAPE group bacteria modified their cytotoxicity against JEG-3 and K-562 cell lines and their and mRNA levels. Our findings indicate that bacteria can modify NK cell features and their interactions with other cells in the microenvironment.

自然杀伤细胞（NK细胞）的功能可受环境刺激调节。然而，细菌成分在这种调节中的作用仍是一个正在进行研究的领域。本研究调查了细菌上清液在共培养后如何影响NK细胞功能，包括细胞毒性、表型、细胞因子和mRNA产生。这些参数通过流式细胞术和逆转录聚合酶链反应（RT-PCR）进行测量。我们发现NK细胞表达Toll样受体2（TLR2）和Toll样受体5（TLR5），并且暴露于ESKAPE组细菌的上清液会改变它们对JEG-3和K-562细胞系的细胞毒性以及它们的和 mRNA水平。我们的研究结果表明，细菌可改变NK细胞的特征及其在微环境中与其他细胞的相互作用。