Innovation Platform of Molecular Biology, College of Landscape and Art, Henan Agricultural University, Zhengzhou, China.
Innovation Platform of Molecular Biology, College of Landscape and Art, Henan Agricultural University, Zhengzhou, China.
Genomics. 2020 Nov;112(6):5005-5011. doi: 10.1016/j.ygeno.2020.09.027. Epub 2020 Sep 12.
Identifying physiological and transcriptomic changes can provide insights into the effects of drier air humidity stress on plants. In this study, we selected 6-month-old seedlings of Pterocarya stenoptera as study materials and used physiological index detection and transcriptome sequencing to investigate the adaptation mechanism of P. stenoptera in response to drier air humidity stress. Proline content, and superoxide dismutase and peroxidase activities did not increase significantly under drier air humidity stress. The physiological results showed that the drier air humidity stress only had slight effects on P. stenoptera. However, transcriptome sequencing showed that P. stenoptera initiated a series of metabolic pathways including L-phenylalanine catabolic process, NAD biosynthetic process, ATP biosynthetic process, and thiamine metabolism under drier air humidity stress. The enriched Kyoto Encyclopedia of Genes and Genomes results at 2 and 4 weeks under the drier air humidity stress showed that the genes THI1 and THIC in thiamine metabolism exhibited significantly differential expression. Previous studies confirmed that the two genes can improve drought tolerance. Our results implicitly indicated that exogenous thiamine might improve drought tolerance and alleviate the yellowing of the P. stenoptera leaves. Our study provides insights into the adaptation mechanism of P. stenoptera in response to drier air humidity stress and important clues into the cultivation and management of P. stenoptera in northern cities in China.
鉴定生理和转录组变化可以深入了解干燥空气湿度胁迫对植物的影响。本研究以 6 个月大的楸树幼苗为研究材料，采用生理指标检测和转录组测序技术，研究了楸树对干燥空气湿度胁迫的适应机制。脯氨酸含量、超氧化物歧化酶和过氧化物酶活性在干燥空气湿度胁迫下并未显著增加。生理结果表明，干燥空气湿度胁迫仅对楸树产生轻微影响。然而，转录组测序结果表明，在干燥空气湿度胁迫下，楸树启动了一系列代谢途径，包括 L-苯丙氨酸分解代谢过程、NAD 生物合成过程、ATP 生物合成过程和硫胺素代谢。在干燥空气湿度胁迫下 2 周和 4 周的京都基因与基因组百科全书富集结果表明，硫胺素代谢中的 THI1 和 THIC 基因表现出显著的差异表达。先前的研究证实，这两个基因可以提高耐旱性。我们的结果暗示外源性硫胺素可能提高耐旱性并减轻楸树叶片的黄化。本研究为楸树应对干燥空气湿度胁迫的适应机制提供了深入了解，并为中国北方城市的楸树栽培和管理提供了重要线索。
