IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina; Cátedra de Fisiología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina.
IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina.
Anim Reprod Sci. 2020 Oct;221:106595. doi: 10.1016/j.anireprosci.2020.106595. Epub 2020 Sep 6.
Zinc (Zn) has important functions in mammalian reproductive processes. In cattle, Zn status can be classified as deficient, marginal, and adequate, depending on the plasma Zn concentration. In addition, Zn deficiency can lead to reproductive failure. The aim of this study was to investigate the effect of maternal Zn status at the beginning of a fixed-time artificial insemination (FTAI) treatment regimen on pregnancy rate in cattle, and evaluate the effect of supplementing in vitro fertilization (IVF) medium with Zn concentrations within the reference range for Zn status on sperm quality and IVF performance. Pregnancy rates of animals with marginal and adequate Zn status did not differ, and there were no Zn-deficient animals detected. Supplementation of 0.8 μg/mL Zn to IVF medium enhanced progressive motility, sperm viability, functional sperm membrane integrity (HOST), acrosomal integrity and sperm-zona binding, without modifying pronuclear formation, or development of embryos to the cleavage or blastocyst stage after IVF. In conclusion, the present results indicate pregnancy rates are not associated with maternal Zn status at the beginning of the FTAI treatment regimen if Zn status is marginal or adequate. Furthermore, supplementation of IVF medium with Zn at concentrations which is considered adequate for Zn status in cattle led to improved sperm quality, without having effects on embryo development in cattle.
锌（Zn）在哺乳动物生殖过程中具有重要功能。在牛中，根据血浆 Zn 浓度可将 Zn 状态分为缺乏、边缘和充足。此外，Zn 缺乏可导致生殖失败。本研究旨在探讨固定时间人工授精（FTAI）治疗开始时母体 Zn 状态对牛妊娠率的影响，并评估在体外受精（IVF）培养基中补充 Zn 浓度处于 Zn 状态参考范围对精子质量和 IVF 性能的影响。具有边缘和充足 Zn 状态的动物的妊娠率没有差异，并且没有检测到 Zn 缺乏的动物。向 IVF 培养基中补充 0.8μg/mL Zn 可提高精子的运动能力、活力、功能精子膜完整性（HOST）、顶体完整性和精子-透明带结合能力，而不会改变原核形成或胚胎在 IVF 后的卵裂或囊胚阶段的发育。综上所述，如果 Zn 状态为边缘或充足，那么 FTAI 治疗开始时母体 Zn 状态与妊娠率无关。此外，在 IVF 培养基中补充 Zn 的浓度被认为是牛的 Zn 状态充足时，可改善精子质量，而对牛胚胎的发育没有影响。
