Anchordoquy J P, Anchordoquy J M, Pascua A M, Nikoloff N, Peral-García P, Furnus C C
IGEVET - Instituto de Genética Veterinaria "Prof. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina; Cátedra de Fisiología, Laboratorio de Nutrición Mineral, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina.
IGEVET - Instituto de Genética Veterinaria "Prof. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CP 1900, La Plata, Buenos Aires, Argentina.
Theriogenology. 2017 Jul 15;97:124-133. doi: 10.1016/j.theriogenology.2017.04.037. Epub 2017 Apr 26.
Adequate dietary intake of copper (Cu) is required for normal reproductive performance in cattle. The objective of this study was to investigate the pregnancy rates from cattle with deficient, marginal and adequate Cu plasma concentration at the beginning of artificial insemination protocol. Moreover, we determined Cu concentrations present in bovine oviductal fluid (OF), and the effects of Cu on fertilizing ability of bovine spermatozoa. Also, the presence of Cu transporter, SLC31A1 (also known as CTR1), in spermatozoa and in vitro matured oocyte were investigated. We found no differences in pregnancy rates among animals with adequate, marginal, and deficient Cu concentrations measured in plasma at the beginning of fixed-time artificial insemination (FTAI) protocol. Copper concentrations in OF were 38.3 ± 2.17 μg/dL (mean ± SEM) regardless of cupremia levels. The addition of 40 μg/dL Cu to IVF medium enhanced total and progressive motility, sperm viability, functional sperm membrane integrity (HOST), sperm-zona binding, and pronuclear formation. On the other hand, the presence of Cu in IVF medium did not modify acrosome integrity and cleavage rates after IVF, but impaired blastocyst rates. Cu transporter SLC31A1 was detected in bovine spermatozoa in the apical segment of acrosome, and in the oocyte matured in vitro. In conclusion, the results obtained in the present study determined that cupremia levels at the beginning of FTAI protocol did not influence the pregnancy rates at 60 d after insemination. The presence of CTR1 in bovine mature oocyte and spermatozoa, as well as the beneficial effect of Cu on sperm quality would suggest an important role of this mineral during the fertilization process.
牛正常繁殖性能需要充足的膳食铜（Cu）摄入。本研究的目的是调查人工授精方案开始时血浆铜浓度不足、处于临界值和充足的牛的妊娠率。此外，我们测定了牛输卵管液（OF）中的铜浓度，以及铜对牛精子受精能力的影响。同时，研究了铜转运蛋白SLC31A1（也称为CTR1）在精子和体外成熟卵母细胞中的存在情况。我们发现，在定时人工授精（FTAI）方案开始时，血浆中铜浓度充足、处于临界值和不足的动物之间，妊娠率没有差异。无论血铜水平如何，OF中的铜浓度均为38.3±2.17μg/dL（平均值±标准误）。在体外受精（IVF）培养基中添加40μg/dL铜可提高精子的总活力和前向运动能力、精子活力、功能性精子膜完整性（HOST）、精子与透明带的结合以及原核形成。另一方面，IVF培养基中铜的存在不会改变IVF后的顶体完整性和卵裂率，但会损害囊胚率。在牛精子顶体顶端段和体外成熟的卵母细胞中检测到了铜转运蛋白SLC31A1。总之，本研究结果表明，FTAI方案开始时的血铜水平不会影响授精后60天的妊娠率。CTR1在牛成熟卵母细胞和精子中的存在，以及铜对精子质量的有益作用，表明这种矿物质在受精过程中具有重要作用。
